Charcoal stabilization of phenyl phosphates
First Claim
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1. A kit for phosphatase-phenyl phosphate reactions comprising separately (i) a phosphatase and (ii) a stabilized, colorless solubilized phenyl phosphate, wherein the stabilized, colorless solubilized phenyl phosphate comprises phenyl phosphate, a buffer, and charcoal.
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Abstract
The invention discloses methods, compositions and kits for stabilizing a solubilized phenyl phosphate, preferably paranitrophenyl phosphate (PNPP), using charcoal. Also disclosed are methods, compositions and kits for recycling solubilized phenyl phosphate, preferably PNPP, that has an absorbance of less than 0.1 when measured at 405 nm due to non-enzymatic hydrolysis.
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21 Claims
- 1. A kit for phosphatase-phenyl phosphate reactions comprising separately (i) a phosphatase and (ii) a stabilized, colorless solubilized phenyl phosphate, wherein the stabilized, colorless solubilized phenyl phosphate comprises phenyl phosphate, a buffer, and charcoal.
- 17. A kit for recycling a solubilized phenyl phosphate having an absorbance over 0.1 OD due to non-enzymatic hydrolysis when measured at 400 to 410 nm comprising a solubilized phenyl phosphate which has been colored due to non-enzymatic hydrolysis and a stabilizing amount of charcoal.
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