Brush-like structured surface of poly(ethylene oxide) having elevated density

• US 7,214,500 B2
• Filed: 03/07/2003
• Issued: 05/08/2007
• Est. Priority Date: 03/11/2002
• Status: Expired due to Fees

• Current Assignee: Toudai Tlo, Ltd.
• Original Assignee: Toudai Tlo, Ltd.
• Inventors: Kataoka, Kazunori, Nagasaki, Yukio, Takahashi, Tadahito, Kato, Miki, Otsuka, Hidenori, Uchida, Katsumi
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): Haq; Shafiqul
• Application Number:
  US10/507,303
• Publication Number:
  US 20050142028A1
• Time in Patent Office: 1,523 Days
• Field of Search: 436/525, 436/527, 525/535, 528/370, 528/373, 528/374, 435/7.92, 435/287.2, 435/7.1, 425/78.31, 530/402
• US Class Current: 435/7.92
• CPC Class Codes: C08G 65/329 with organic compounds
• CPC Class Codes: C08G 65/334 containing sulfur
• CPC Class Codes: C08G 65/336 containing silicon
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A surface on which adsorption of protein (e.g., BSA) is markedly suppressed is provided, by contacting solutions of polymers whose chief component is poly(ethylene oxide) having binding sites to sensor surfaces with biosensor surfaces plural times to link the polymers.

Claims

1. A method for preparing a biosensor chip comprising two groups of polymers expressed by the following general formula (I) which are linked onto the sensor chip surface via the A-moiety present at one end of said polymers:

general formula (I)

A-(CH₂)_p-L₁-(CH₂C₂O)_n-L₂-X 

 

(I)

in which

A stands for HS—

group,

L₁ stands for a first linker or valence bond,

L₂ stands for a second linker or valence bond,

X stands for hydrogen, a functional group, protected functional group or ligand,

p is an integer of 2–

12, and

n is an integer of, on the average, 10–

10,000,

in which one group of said polymers have an integer, n, as an average value, of 50–

10,000

and the other group of said polymers have an integer, n, as an average value less than that of said one group by at least 10, which comprises,

(A) a step of contacting an aqueous solution of said one group of polymers expressed by a general formula (Ia);

HS—

(CH₂)_p-L₁-(CH₂CH₂O)_n-L₂-X 

 

(Ia)

in which L₁, L₂, X, p and n have the same significations to those as defined as to the general formula (I),

with the sensor chip surface of a metal selected from the group consisting of gold, silver, copper and aluminum, under the conditions sufficient to link a prescribed amount of said polymer to said metallic surface, and thereafter washing away the unlinked polymer;

(B) a step of subsequently contacting an aqueous solution of said other group of polymers of general formula (Ia) which have an integer, n, as an average value, less than that of said one group of polymers in step (A) by at least 10, with the sensor chip surface which has undergone the above step A, under the conditions sufficient to link said polymer to said surface, and thereafter washing away the unlinked polymer; and

(C) repeating the above step (A), (B) or both steps (A) and (B) plural times to obtain said biosensor chip.

2. A method for treating the surface of a material selected from the group consisting of glass, semi-conductor, ceramic, metal oxide and alloy oxide, where two groups of polymers expressed by the following general formula (I) are linked onto the surface via the A-moiety present at one end of said polymers:

A-(CH₂)_p-L₁-(CH₂CH₂O)_n-L₂-X 

 

general formula (I)

in which

A stands for