Method for screening crystallization conditions in solution crystal growth
First Claim
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1. A method of screening protein crystal growth conditions comprising:
- dispensing a protein solution into protein solution microchambers of a microarray and separately dispensing precipitate solutions into precipitate solution microchambers of said microarray, said microchambers having a volume from about 0.001 nl to about 250 nl;
allowing liquid—
liquid communication between protein solution microchambers and precipitate solution microchambers; and
observing said microchambers for protein crystal growth or protein precipitation.
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Abstract
A method of screening protein crystal growth conditions on a nano or meso scale comprises providing a micro-electromechanical chip with a plurality of micro-chambers in the micro-electromechanical chip. A protein solution is placed into the micro-chambers by an automated jet type dispensing means with nano or meso scale precision. The protein crystal growth conditions of each of the micro-chambers is adjusted so that the protein crystal growth conditions of each of the micro-chambers differs. Crystallization of the protein solution in the micro-chambers is effected. Protein crystal growth in the micro-chambers is then observed.
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16 Claims
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1. A method of screening protein crystal growth conditions comprising:
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dispensing a protein solution into protein solution microchambers of a microarray and separately dispensing precipitate solutions into precipitate solution microchambers of said microarray, said microchambers having a volume from about 0.001 nl to about 250 nl; allowing liquid—
liquid communication between protein solution microchambers and precipitate solution microchambers; andobserving said microchambers for protein crystal growth or protein precipitation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 13, 14, 15, 16)
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10. A method of screening protein crystal growth conditions comprising:
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transferring samples comprising a volume from about 0.001 nl to about 250 nl of a protein solution into microchambers in a platform; providing a precipitate solution that is in liquid—
liquid communication with the protein solution;controlling the protein crystal growth conditions of the samples; and observing a protein precipitate or protein crystals in the microchambers. - View Dependent Claims (11, 12)
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Specification