Tripeptide prodrug compounds

• US 7,214,663 B2
• Filed: 06/11/2001
• Issued: 05/08/2007
• Est. Priority Date: 06/14/2000
• Status: Expired due to Term

• Current Assignee: ER Squibb & Sons LLC (Bristol-Myers Squibb Company)
• Original Assignee: Medarex Incorporated (Bristol-Myers Squibb Company)
• Inventors: Gangwar, Sanjeev, Lobl, Thomas J., Dubois, Vincent, Yarranton, Geoffrey T., Bebbington, Christopher R., Trouet, Andre, Pickford, Lesley B., Nieder, Matthew H.
• Primary Examiner(s): Gupta; Anish
• Application Number:
  US10/311,519
• Publication Number:
  US 20030181359A1
• Time in Patent Office: 2,157 Days
• Field of Search: None
• US Class Current: 514/1.3
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 47/65 Peptidic linkers, binders or spacers, e.g. peptidic enzyme-labile linkers
• CPC Class Codes: A61K 47/67 Enzyme prodrug therapy, e.g. gene directed enzyme drug therapy [GDEPT] or VDEPT
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07K 5/0202 containing the structure -NH-X-X-C(=0)-, X being an optionally substituted carbon atom or a heteroatom, e.g. beta-amino acids
• CPC Class Codes: C07K 5/0808 the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
• CPC Class Codes: C07K 5/081 the side chain containing O or S as heteroatoms, e.g. Cys, Ser
• CPC Class Codes: C07K 5/0812 and aromatic or cycloaliphatic

Abstract

The prodrug of the invention is a modified form of a therapeutic agent and comprises a therapeutic agent, an oligopeptide of three amino acids, a stabilizing group and, optionally, a linker group. The prodrug is cleavable by a trouase enzyme such as Thimet oligopeptidase. Also disclosed are methods of making and using the prodrug compounds.

Claims

1. A compound comprising:

(1) a therapeutic agent capable of entering a target cell,

(2) an oligopeptide of the formula AA³-AA²-AA¹ wherein the oligopeptide is selected from the group consisting of;

Leu-Ala-GIy (SEQ ID NO;

     10), and Leu-Tyr-Leu (SEQ ID NO;

     13);

(3) a stabilizing group;

(4) optionally, a linker group not cleavable by TOP,

wherein the oligopeptide is directly linked to the stabilizing group at a first attachment site of the oligopeptide and the oligopeptide is directly linked to the therapeutic agent or indirectly linked through the linker group to the therapeutic agent at a second attachment site of the oligopeptide,

wherein the stabilizing group hinders cleavage of the oligopeptide by enzymes present in whole blood, and wherein the compound is cleavable by TOP.

2. The compound of claim 1 wherein the stabilizing group is a dicarboxylic or higher order carboxylic acid.

3. The compound of claim 1 wherein the stabilizing group is selected from the group consisting of:

succinic acid, adipic acid, glutaric acid, phthalic acid, diglycolic acid, fumaric acid, naphthalene dicarboxylic acid, pyroglutamic acid, acetic acid, 1-naphthylcarboxylic acid, 2-naphthylcarboxylic acid, 1,8-naphthyl dicarboxylic acid, aconitic acid, carboxycinnamic acid, triazole dicarboxylic acid, gluconic acid, 4-carboxyphenyl boronic acid, polyethylene glycolic acid, butane disulfonic acid, nipecotic acid, isonipecotic acid, and maleic acid.

4. The compound of claim 1 wherein the stabilizing group is a non-genetically encoded amino acid having four or more carbons.

5. The compound of claim 1 wherein the stabilizing group is one of aspartic acid linked to the oligopeptide at the β

-carboxy group of the aspartic acid or glutamic acid linked to the oligopeptide at the γ

-carboxy group of the glutamic acid.

6. The compound of claim 1 wherein the stabilizing group is negatively charged or neutral.

7. The compound of claim 1 wherein the stabilizing group reduces interaction between the compound and endothelial cells that line blood vessels when administered to the patient.

8. The compound of claim 1 wherein TOP cleaves the linkage between AA³ and AA² of the oligopeptide.

9. The compound of claim 1 wherein the compound is cleaved by TOP under an experimental condition at a test rate of cleavage of 10–

80% of a standard rate of cleavage, the standard rate of cleavage tested on a test standard by TOP under the experimental condition, the test standard consisting of a conjugate of Suc-β

Ala-Leu-Ala-Leu and the therapeutic agent.

10. The compound of claim 9 wherein the test rate of cleavage is 30–

65% of the standard rate of cleavage.

11. The compound of claim 1 wherein the therapeutic agent is selected from the group consisting of the group consisting of Alkylating Agents, Antiproliferative agents, Tubulin Binding agents, Vinca Alkaloids, Enediynes, Podophyllotoxins or Podophyllotoxin derivatives, the Pteridine family of drugs, Taxanes, Anthracyclines, Dolastatins, Topoiosomerase inhibitors, Maytanisoids and Platinum coordination complex chemotherapeutic agents, derivatives of the foregoing and analogs of the foregoing.

12. The compound of claim 1 wherein the therapeutic agent is selected from the group consisting of Doxorubicin, Daunorubicin, Vinblastine, Vincristine, Calicheamicin, Etoposide, Etoposide phosphate, CC-1065, Duocarmycin, KW-2189, Methotrexate, Methopterin, Aminopterin, Dichloromethotrexate, Docetaxel, Paclitaxel, Epithiolone, Combretastatin, Combretastatin A4 Phosphate, Dolastatin 10, Dolastatin 11, Dolastatin 15, Topotecan, Camptothecin, Mitomycin C, Porfiromycin, 5-Fluorouracil, 6-Mercaptopurine, Fludarabine, Tamoxifen, Cytosine arabinoside, Adenosine Arabinoside, Colchicine, Carboplatin, cis-Platin, Mitomycin C, Bleomycin, Melphalan, Chloroquine, Cyclosporin A, Maytansine, and derivatives of the foregoing, and analogs of the foregoing.

13. The compound of claim 1 wherein the target cell is a tumor or inflammatory cell.

14. The compound of claim 1 being a prodrug having an active portion, wherein the active portion of the prodrug is more capable of entering the target cell after cleavage by TOP than prior to cleavage by TOP, the active portion including at least the therapeutic agent.

15. The compound of claim 14 wherein the active portion of the prodrug consists of the therapeutic agent.

16. The compound of claim 14 wherein the active portion of the prodrug includes the therapeutic agent and at least the linker group.

17. The compound of claim 14 wherein the active portion of the prodrug includes the therapeutic agent and AA¹ of the oligopeptide.

18. The compound of claim 17 wherein the active portion of the prodrug further comprises AA² of the oligopeptide linked to AA¹.

19. The compound of claim 1 wherein the oligopeptide is directly linked to the therapeutic agent.

20. The compound of claim 1 wherein the oligopeptide sequence is indirectly linked to the therapeutic agent at the second attachment site of the oligopeptide via a linker group, the linker group selected from the group consisting of amino caproic acid, hydrazide group, an ester group, an ether group, and a sulphydryl group.

21. The compound of claim 1 wherein the compound is selected from the group consisting of, Suc-Leu-Ala-Gly-Dox, Suc-Leu-Tyr-Leu-Dox.

22. The compound of claim 1 wherein the compound is resistant to cleavage by CD10.

23. A compound comprising:

(1) a therapeutic agent capable of entering a target cell,

(2) an oligopeptide peptide selected from the group consisting of;

Leu-Ala-GIy (SEQ ID NO;

     10), and Leu-Tyr-Leu (SEQ ID NO;

     13);

3) a stabilizing group, the stabilizing group selected from;

(a) a dicarboxylic or higher order carboxylic acid,

(b) a non-genetically encoded amino acid having four or more carbons, or

(c) one of aspartic acid linked to the oligopeptide at the β

-carboxy group of the aspartic acid or glutamic acid linked to the oligopeptide at the γ

-carboxy group of the glutamic acid, and

(4) optionally, a linker group not cleavable by TOP,

wherein the oligopeptide is directly linked to the stabilizing group at a first attachment site of the oligopeptide and the oligopeptide is directly linked to the therapeutic agent or indirectly linked through the linker group to the therapeutic agent at a second attachment site of the oligopeptide,

wherein the stabilizing group hinders cleavage of the oligopeptide by enzymes present in whole blood, and wherein the compound is cleavable by TOP.

24. The compound of claim 23 wherein the stabilizing group is selected from the group consisting of:

succinic acid, adipic acid, glutaric acid, phthalic acid, diglycolic acid, fumaric acid, naphthalene dicarboxylic acid, pyroglutamic acid, acetic acid, 1-naphthylcarboxylic acid, 2-naphthylcarboxylic acid, 1,8-naphthyl dicarboxylic acid, aconitic acid, carboxycinnamic acid, triazole dicarboxylic acid, gluconic acid, 4-carboxyphenyl boronic acid, polyethylene glycolic acid, butane disulfonic acid, nipecotic acid, isonipecotic acid, and maleic acid.

25. The compound of claim 23 wherein the compound is resistant to cleavage by CD10.

26. A pharmaceutical composition comprising:

(1) a compound comprising;

(a) a therapeutic agent capable of entering a target cell,

(b) an oligopeptide peptide selected from the group consisting of;

Leu-Ala-GIy (SEQ ID NO;

     10), and Leu-Tyr-Leu (SEQ ID NO;

     13);

(c) a stabilizing group, the stabilizing group selected from;

(d) optionally, a linker group not cleavable by TOP,

wherein the oligopeptide is directly linked to the stabilizing group at a first attachment site of the oligopeptide and the oligopeptide is directly linked to the therapeutic agent or indirectly linked through the linker group to the therapeutic agent at a second attachment site of the oligopeptide, wherein the stabilizing group hinders cleavage of the oligopeptide by enzymes present in whole blood, and wherein the compound is cleavable by TOP, and

(2) a pharmaceutically acceptable carrier.