Surface modified protein microparticles

• US 7,217,410 B2
• Filed: 06/17/2003
• Issued: 05/15/2007
• Est. Priority Date: 06/17/2003
• Status: Active Grant

• Current Assignee: Board of Trustees of The University of Illinois
• Original Assignee: Board of Trustees of The University of Illinois
• Inventors: Boppart, Stephen A., Marks, Daniel L., Toublan, Farah Jean-Jacques, Suslick, Kenneth S.
• Primary Examiner(s): JONES, DAMERON LEVEST
• Application Number:
  US10/463,833
• Publication Number:
  US 20040258759A1
• Time in Patent Office: 1,428 Days
• Field of Search: 424/1.11, 424/1.29, 424/1.33, 424/1.37, 424/1.65, 424/9.1, 424/9.3, 424/9.4, 424/9.5, 424/9.6, 424/9.7, 424/9.8, 428/402, 428/402.2
• US Class Current: 424/1.29
• CPC Class Codes: A61K 49/001 Preparation for luminescence or biological staining

Abstract

A microparticle contains a cross-linked protein shell, and a surface coating.

Claims

1. A microparticle, comprising:

a cross-linked protein shell, and

a covalently-attached surface coating.

2. The microparticle of claim 1, wherein the protein shell contains at least one moiety selected from the group consisting of amine moieties, hydroxyl moieties, carboxylate moieties and sulfhydryl moieties.

3. The microparticle of claim 1, wherein the surface coating comprises polyethylene glycol.

4. The microparticle of claim 3, wherein the surface coating further comprises a ligand for a cell-surface receptor.

5. The microparticle of claim 4, wherein the ligand is folate.

6. The microparticle of claim 3, wherein the surface coating further comprises a second protein.

7. The microparticle of claim 1, wherein the surface coating comprises a second protein.

8. The microparticle of claim 6, wherein the second protein is an antibody.

9. The microparticle of claim 7, wherein the second protein is an antibody.

10. The microparticle of claim 1, wherein the cross-linked protein shell comprises cross-linked albumin.

11. The microparticle of claim 3, wherein the cross-linked protein shell comprises cross-linked albumin.

16. The microparticle of claim 1, further comprising a core comprising a pharmaceutically active compound.

17. The microparticle of claim 1, further comprising a core comprising a member selected from the group consisting of Gd complexes, melanin, colloidal gold, iron oxide, titanium dioxide, and fluorocarbons.

20. The microparticle of claim 1, further comprising a core comprising a liquid crystalline material.

22. The microparticle of claim 1, wherein the cross-linked protein shell comprises at least one cross-linked protein, the protein selected from the group consisting of hemoglobin, pepsin, immunoglobulin, lipase, peroxidase and myoglobin.

12. A microparticle, comprising a cross-linked protein shell, and a surface coating comprising particles.

13. The microparticle of claim 12, wherein the cross-linked protein shell comprises cross-linked albumin.

14. The microparticle of claim 12, wherein the particles are colloidal particles selected from the group consisting of ceramics, metals and semiconductors.

15. The microparticle of claim 12, wherein the particles are selected from the group consisting of melanin, carbon, gold, and silica.

18. The microparticle of claim 12, further comprising a core comprising a pharmaceutically active compound.

19. The microparticle of claim 12, further comprising a core comprising a member selected from the group consisting of Gd complexes, melanin, colloidal gold, iron oxide, titanium dioxide, and fluorcarbons.

21. The microparticle of claim 12, further comprising a core comprising a liquid crystalline material.

23. The microparticle of claim 12, wherein the cross-linked protein shell comprises at least one cross-linked protein, the protein selected from the group consisting of hemoglobin, pepsin, immunoglobulin, lipase, peroxidase and myoglobin.

24. The microparticle of claim 12, wherein the cross-linked protein shell encapsulates a core.

25. A microparticle, comprising:

a core, and a shell encapsulating the core, and

a surface coating on the shell,

wherein the shell comprises proteins that are cross-linked together, and

the surface coating comprises particles.

26. The microparticle of claim 25, wherein the proteins comprises at least one member selected from the group consisting of albumin, hemoglobin, pepsin, immunoglobin, lipase, peroxidase, and myoglobin.

27. The microparticle of claim 25, further comprising at least one moiety selected from the group consisting of amine moieties, hydroxyl moieties, carboxylate moieties and sulfhydryl moieties.

28. The microparticle of claim 25, wherein the proteins comprise a chemical modification.

29. The microparticle of claim 28, wherein the chemical modification comprises a carbodiimide.