Microwell biochip
First Claim
1. A device for holding a liquid solution and allowing the reaction thereof with immobilized biological material as a part of an assay, which device comprises:
- a plate in which there are formed a group of wells which extend completely therethrough, wherein the walls of said wells are substantially liquid impervious,a microporous material closing the bottom of each said well, andat least one spot of a polymer attached to the upper surface of said microporous material at the bottom of each of a plurality of said wells, which spot comprises a crosslinked hydrogel polymer having biological material so immobilized on or within the polymer as to be contactable by a liquid supplied to said well and which spot covers only a portion of the bottom surface of each well, leaving a substantial portion through which drainage can be effected.
1 Assignment
0 Petitions
Accused Products
Abstract
Microwell biochips (11) are formed from a thin flat plate (13) of polymeric material having a plurality of regularly spaced holes (15) that extend completely therethrough and create microwells. The lower end of each hole is closed by a microporous, hydrophobic, polymeric membrane (17) laminated to the undersurface of the plate which retains an aqueous test solution in the wells until a vacuum is applied to the undersurface thereof to effect draining of the solution and of any wash solution that might be subsequently added. A spot of polymerizing isocyanate-functional hydrogel is applied generally centrally to the porous membrane surface at the bottom of each well in a manner so as to cover only a minor portion of the surface and out of contact with the well sidewalls, thus leaving substantial surface area through which drainage can be readily effected. Biological capture agents are associated with the polymerizing hydrogel so as to become immobilized as a part thereof.
132 Citations
18 Claims
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1. A device for holding a liquid solution and allowing the reaction thereof with immobilized biological material as a part of an assay, which device comprises:
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a plate in which there are formed a group of wells which extend completely therethrough, wherein the walls of said wells are substantially liquid impervious, a microporous material closing the bottom of each said well, and at least one spot of a polymer attached to the upper surface of said microporous material at the bottom of each of a plurality of said wells, which spot comprises a crosslinked hydrogel polymer having biological material so immobilized on or within the polymer as to be contactable by a liquid supplied to said well and which spot covers only a portion of the bottom surface of each well, leaving a substantial portion through which drainage can be effected. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A multiwell device for holding liquid solutions in the wells and allowing reaction to occur between the solution and immobilized biological material in the wells as a part of an assay, which device comprises:
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a flat plate in which there are formed a plurality of wells by holes which extend completely through the thickness of said plate, wherein the sidewalls of said holes provide walls of said wells which are substantially liquid impervious, a microporous material closing the bottom of each said hole, at least one three-dimensional spot of a crosslinked hydrogel polymer attached to only a portion of an upper surface area of said microporous material at the bottom of each of a plurality of said wells so that drainage can be effected through the portion of said surface area without said hydrogel polymer, and biological material so immobilized on or within the hydrogel polymer as to be contactable by liquid supplied to said well.
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Specification