Method and apparatus for biosensor spectral shift detection
First Claim
1. A method for determining biosensor resonance peak offsets comprising the steps:
- (i) determining a reference spectrum and a sample spectrum, the reference spectrum having a magnitude and a slope; and
,(ii) determining a peak resonance offset in response to the slope of the reference spectrum.
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Accused Products
Abstract
Performing high-resolution determination of the relative shift of the spectral properties of a biosensor. The shift in the resonance peak of the biosensor is indicative of the amount of material bound to the surface of the biosensor. A preferred biosensor is a Guided Mode Resonant Filter Biosensor (GMRFB). In one aspect of the invention, curve fitting is used to determine the relative location of the spectrum of the unexposed biosensor with respect to those spectra that are altered (e.g., shifted) by the presence of materials bound to the surface of the biosensor. In an alternative embodiment, the cross correlation function is used to detect spectral peak offsets between a reference spectrum and a spectrum measured from an exposed biosensor. In yet another alternative, maximal likelihood estimation techniques are used to determine the spectral shift or offs.
178 Citations
14 Claims
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1. A method for determining biosensor resonance peak offsets comprising the steps:
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(i) determining a reference spectrum and a sample spectrum, the reference spectrum having a magnitude and a slope; and
,(ii) determining a peak resonance offset in response to the slope of the reference spectrum. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification