Compositions comprising genome segments and methods of using the same
First Claim
1. A method of treating a human individual who has a disease or disorder associated with exposure to ionizing radiation comprising the step ofadministering to the individual who has been exposed to ionizing radiation a therapeutically effective amount of a plurality of polynucleotide molecules that are free of vector sequences, whereinthe plurality of polynucleotide molecules collectively comprises an essentially complete human genome derived from autologous DNA collected from the individual before the individual is exposed to the ionizing radiation;
- and each of the plurality of polynucleotide molecules having about 100–
3000 nucleotides.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods of treating an individual who has a disease or disorder associated with one or more genetic mutations or undesirable alleles in genomic DNA of the individual, or preventing an individual from developing a disease or disorder associated with one or more genetic mutations or undesirable alleles in genomic DNA of the individual, by replacing a segment of genomic DNA that has a mutated sequence or undesirable allele with a corresponding segment of DNA that has a non-mutated sequence or desirable allele, are disclosed. Methods of inducing tolerance and preventing transplant rejection in a recipient, methods of inducing tolerance and or reducing allergies are disclosed and method of increasing fertility in a woman are disclosed. Each of the methods comprises replacing a segment of genomic DNA in an individual with a corresponding segment of DNA from another. An apparatus for doing large scale PCR preparations is disclosed. Pharmaceutical compositions that comprise a plurality of polynucleotide molecules which collectively comprise an essentially complete genome in polynucleotide molecules having about 100–3000 nucleotides are disclosed.
-
Citations
8 Claims
-
1. A method of treating a human individual who has a disease or disorder associated with exposure to ionizing radiation comprising the step of
administering to the individual who has been exposed to ionizing radiation a therapeutically effective amount of a plurality of polynucleotide molecules that are free of vector sequences, wherein the plurality of polynucleotide molecules collectively comprises an essentially complete human genome derived from autologous DNA collected from the individual before the individual is exposed to the ionizing radiation; - and each of the plurality of polynucleotide molecules having about 100–
3000 nucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- and each of the plurality of polynucleotide molecules having about 100–
Specification