Film based addressable programmable electronic matrix articles and methods of manufacturing and using the same

• US 7,220,344 B2
• Filed: 07/23/2002
• Issued: 05/22/2007
• Est. Priority Date: 11/18/1999
• Status: Expired due to Fees

• Current Assignee: 3M Innovative Properties Company (3M Company)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Bentsen, James Gregory, Biernath, Rolf Werner
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US10/200,829
• Publication Number:
  US 20020195345A1
• Time in Patent Office: 1,764 Days
• Field of Search: 204/600, 204/606, 204/616, 204/450, 204/456, 204/466
• US Class Current: 204/450
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: G01N 27/44782 of a plurality of samples
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

An electronic device adapted for performing molecular biological processes. The device includes a flexible polymeric substrate having a first surface and a second surface. A plurality of microlocations interrupt the first surface, and each of said microlocations include an electrode disposed on the second surface of the flexible substrate. A hydrophilic matrix is positioned on the first surface of the flexible substrate and is capable of electrical contact with the electrode.

Claims

1. An electronic device adapted for performing electrophoresis assisted processes, the device comprising:

at least one flexible polymeric substrate having a first surface and a second surface;

the first surface being opposite the second surface;

one or more microlocations interrupting the first surface, each of said microlocations including an electrode disposed on the second surface of the flexible substrate and extending to the microlocation;

the electrode defining the microlocation or forming a bottom of the microlocation; and

a hydrophilic matrix positioned on the microlocation and in electrical contact with at least one of the electrodes.

2. The electronic device according to claim 1, wherein the hydrophilic matrix comprises a biologically receptive gel.

3. The electronic device according to claim 1, wherein the hydrophilic matrix comprises a biologically reactive gel.

4. The electronic device according to claim 1, wherein the electrophoresis assisted processes include biological processes.

5. The electronic device according to claim 1, wherein the electrophoresis assisted processes include molecular biological processes.

6. The electronic device according to claim 1, wherein the flexible polymeric substrate comprises polyimide.

7. The electronic device according to claim 1, wherein the plurality of microlocations interrupting the first surface comprise vias that extend from the first surface into the polymeric substrate.

8. The electronic device according to claim 1, wherein the plurality of microlocations interrupting the first surface further comprise a raised area of electrically conductive material.

9. The electronic device according to claim 1, wherein the microlocations have a smallest dimension in the plane of the first surface of less than 200 μ

m.

10. The electronic device of claim 1, wherein the hydrophilic matrix composes an azlactone functional polymer.

11. The electronic device according to claim 1, wherein a portion of the first surface, the second surface, or both surfaces is partially masked.

12. The electronic device according to claim 1, further comprising a plurality of conductive traces, each trace connected to at least one of said electrodes.

13. The electronic device according to claim 1 additionally comprising at least one electroresistive layer positioned adjacent to at least one electrode.

14. The electronic device according to claim 13 wherein at least one electroresistive layer can function to increase the temperature of at least one electrode.

15. An electronic device adapted for performing electrophoresis assisted processes, the device comprising:

at least a first flexible polymeric substrate having a first surface and a second surface;

the first surface being opposite the second surface;

at least a second flexible polymeric substrate, having a first surface adjacent the second surface of the first polymeric substrate;

at least one microlocation penetrating the first polymeric substrate and the second polymeric substrate in registration, each of the microlocations including an electrode disposed on the second surface of the second polymeric substrate;

the electrode forming a bottom of the microlocation; and

a hydrophilic matrix positioned on the microlocation and in electrical contact with at least one of the electrodes on the second surface of the second polymeric substrate.

16. An electronic device adapted for performing electrophoresis assisted processes, the device comprising:

at least one flexible polymeric substrate having a first major surface;

one or more microlocations disposed on the first major surface of the substrate, each of the microlocations including an electrode;

the electrode defining the microlocation or forming a bottom of the microlocation; and

a hydrophilic matrix positioned on the microlocation and in electrical contact with at least one of the electrodes disposed thereon.

17. The electronic device according to claim 16, further comprising a fluid, handling architecture sealingly engaged with said first surface in registration with said microlocations.

18. The electronic device according to claim 17 wherein said fluid handling architecture comprises a micromolded plastic fluid handling architecture.

19. A flexible polymeric sheet containing a plurality of addressable programmable electronic matrix (APEX) arrays, wherein the plurality of arrays are configured on a substantially continuous sheet, each array adapted for performing an electrophoresis-assisted process, each of the arrays comprising:

a flexible polymeric substrate having a first surface and a second surface;

the first surface being opposite the second surface;

a hydrophilic matrix including an azlactone-functional polymer positioned on the first surface of the flexible polymeric substrate; and

a plurality of microlocations interrupting the first surface, each of said microlocations including an electrode disposed on the second surface of the flexible substrate and extending to the microlocation;

the electrode defining the microlocation or forming a bottom of the microlocation.

20. The flexible polymeric sheet according to claim 19, wherein the flexible polymeric substrate comprises polyimide.

21. The flexible polymeric sheet according to claim 19, further comprising:

a second surface on the flexible polymeric substrate; and

an electrode disposed on the second surface;

wherein an electrode is located adjacent to each of said microlocations and the hydrophilic matrix is in electrical contact with the electrode.

22. The flexible polymeric sheet according to claim 21 comprising from 1 to 200 APEX arrays.

23. A biocard comprising a flexible polymeric sheet according to claim 22 mated in registration on the first surface with a fluid handling architecture.

24. The biocard according to claim 23, wherein said fluid handling architecture comprises a molded flexible polymeric part.

25. A method of making an electronic device adapted for performing molecular biological processes, the method comprising:

providing a flexible polymeric substrate having a first surface and a second surface;

the first surface being opposite the second surface;

wherein the flexible polymeric substrate is substantially continuous;

forming a plurality of microlocation interrupting the first surface, each of said microlocations including an electrode disposed on the second surface of the flexible substrate and extending to the microlocation;

the electrode defining the microlocation or forming a bottom of the microlocation; and

applying a hydrophilic matrix including an azlactone-functional polymer on at least one of the first surface of the flexible substrate and the microlocations, wherein the hydrophilic matrix makes electrical contact with the electrode.

26. The method according to claim 25, further comprising the step of applying at least one of a biological material and a chemical material to at least one of the microlocations.

27. A method of performing molecular biological processes, the method comprising:

providing an electronic device comprising;

a flexible polymeric substrate having a first and second surface;

the first surface being opposite the second surface;

wherein the flexible polymeric substrate is substantially continuous;

one or more microlocations interrupting the first surface, each of said microlocations including an electrode disposed on the second surface of the flexible substrate and extending to the microlocation;

the electrode defining the microlocation or forming a bottom of the microlocation, and

a hydrophilic matrix including an azlactone-functional polymer positioned on the first surface of the flexible substrate and an electrode disposed on the second surface of the flexible substrate, the hydrophilic matrix positioned such that it is in electrical contact with the electrode;

placing a biological sample on the hydrophilic matrix; and

applying an electrical force to the electrode so as to effect a transfer or transformation of the biological sample.

28. The method according to claim 27 further comprising performing from 1 to 200 molecular biological processes, wherein the processes are carried out according to at least one of simultaneous processes and serial processes.