Disposable sensor for use in measuring an analyte in a gaseous sample

• US 7,220,387 B2
• Filed: 01/28/2004
• Issued: 05/22/2007
• Est. Priority Date: 07/23/2002
• Status: Active Grant

• Current Assignee: Circassia AB
• Original Assignee: Apieron Biosystems Corporation
• Inventors: Chazan, David, Parikh, Bhairavi, Stone, James, Nason, Kevin, Flaherty, Bryan, Kaiser, John, Fay, Johnathan
• Primary Examiner(s): Rosenberger; Richard A.
• Application Number:
  US10/767,709
• Publication Number:
  US 20050083527A1
• Time in Patent Office: 1,210 Days
• Field of Search: 356433-435, 356/437, 422 84- 86
• US Class Current: 422/86
• CPC Class Codes: A61B 5/097 Devices for facilitating collection of breath or for directing breath into or through measuring devices
• CPC Class Codes: G01N 21/783 for analysing gases
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath

Abstract

The present invention is a disposable sensor for use with a device that quantifies analytes in a gaseous sample. It comprises (i) a sensing element, (ii) a means for interfacing the disposable sensor with a gas analysis device, and (iii) a housing. When used with a device that can quantify the concentration of an analyte in a gas sample, the present invention facilitates the use of exhaled breath analysis as a clinical tool.

Claims

1. A method of measuring the concentration of nitric oxide in a range of 5 to 200 ppb in a gas sample of exhaled breath, said method comprising:

providing a first disposable sensor, said first sensor comprising a sensing element comprising cytochrome-c in a sol-gel, wherein said sensing element undergoes a change in the presence of 5 to 200 ppb of nitric oxide;

loading said first sensor into a gas analysis device;

measuring the concentration of nitric oxide in an exhaled breath sample using said first disposable sensor and said gas analysis device;

removing said first disposable sensor from said device; and

installing a second disposable sensor into said device, said second disposable sensor likewise comprising a sensing element comprising cyrochrome-c and a sol-gel and undergoing a change in the presence of 5 to 200 ppb of nitric oxide.

2. The method according to claim 1, wherein said first and second disposable sensors are within disposable housings.

3. The method according to claim 1, wherein said change is a change in an optically quantifiable characteristic.

4. The method according to claim 1, further comprising employing means for ensuring that extraneous signals do not interfere with measurement of nitric oxide.

5. The method according to claim 4, wherein said means for ensuring that extraneous signals do not interfere with measurement comprises two separate channels for analysis of the gas, wherein one of said channels is used for reference analysis.

6. The method according to claim 4, wherein said means for ensuring that extraneous signals do not interfere with measurement comprises using two separate sensing elements for analysis of the gas, wherein one of said elements is used for reference analysis.

7. The method according to claim 1, additionally comprising conditioning the gas sample before measurement.

8. The method according to claim 1, wherein said first disposable sensor uses calibration information associated with said sensor.

9. A device for quantifying the concentration of nitric oxide in a range of 5 to 200 ppb in a gaseous sample of exhaled breath, said device comprising:

a disposable sensing element comprising cytocbrome-c in a sol-gel and having a nitric oxide measurement range of 5 to 200 ppb; and

an interface means for interfacing said disposable sensing element with the remainder of said device.

10. The device of claim 9, wherein said interface means comprises a means for aligning an optical window in said disposable sensing element with an optical transducer on said device.

11. The device of claim 9, wherein said disposable sensing element generates an electrical signal and said interface means comprises a means to align electrical contacts on said disposable sensing element with electrical contacts on the remainder of said device.

12. The sensor according to claim 9, wherein said interface means comprises a slot in said device and a guide in said disposable sensing element.

13. The device of claim 9, further comprising two gas cells, one containing said disposable sensing element and the other containing a second said sensing element and means for selectively removing said nitric oxide from exposure to said second sensing element.

14. A kit for determining the concentration of nitric oxide in a sample of exhaled breath in which said nitric oxide is present in an amount ranging from 5 to 200 ppb, said kit comprising:

a plurality of disposable sensors, wherein said sensors comprise cytochrome-c in a sol-gel having a nitric oxide response range of 5 to 200 ppb;

a gas analysis device for use with said sensors, said device comprising means for receiving exhaled breath and converting said response to a measurable signal.