Biochemical analysis unit and method of producing thereof

• US 7,220,389 B2
• Filed: 05/20/2002
• Issued: 05/22/2007
• Est. Priority Date: 05/21/2001
• Status: Expired due to Fees

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: Nakajima, Kenji, Kohda, Katsuhiro, Hosoi, Yuichi, Kato, Akifumi, Neriishi, Keiko, Eto, Masahiro
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): Counts; Gary W.
• Application Number:
  US10/147,826
• Publication Number:
  US 20020197568A1
• Time in Patent Office: 1,828 Days
• Field of Search: 435/6, 435/7.1, 435/283.1, 435/287.2, 435/287.7, 435/287.8, 435/288.4, 435/288.7, 422/52, 422/56, 422/57, 422/82.05, 422/99, 422/101, 436/518, 436/524, 436/525, 436/164
• US Class Current: 422/535
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00364 Pipettes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/168 Specific optical properties, e.g. reflective coatings
• CPC Class Codes: B01L 3/02 Burettes; Pipettes
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 21/0303 : Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/6428 : Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/76 : Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 2201/0642 : Light traps; baffles
• CPC Class Codes: G01N 23/04 : and forming images of the material

Abstract

The biochemical analysis unit has a base plate and absorptive regions. The absorptive regions are surrounded by the base plate formed of materials which shield a radioactive ray and a light. In the absorptive regions are applied and absorbed specific binding substances to be bound with substances derived from a living organism that are labeled with labeling substances for generating the radioactive ray or the light. The base plate prevents the specific binding substances from penetrating in the other absorptive regions. When an analysis of data of the radioactive ray and the light is carried out, an image of the radioactive ray and the light is generated without noises.

Claims

1. A biochemical analysis unit used for analyzing a data of a radioactive ray and a light, comprising:

a plate member formed of a shielding material which can shield at least one of said radioactive ray and said light, said plate member having a first surface and a second surface;

plural through-holes formed in said plate member;

an absorptive membrane formed of an absorptive material, covering said first surface of said plate member, a part of said absorptive membrane provided in said plural through-holes;

absorptive regions formed of through said through-holes of in said second surface of said plate member;

wherein the absorptive membrane is provided to fill more than half of the volume of each of the plural through-holes.

2. A biochemical analysis unit as described in claim 1, wherein in said absorptive regions are absorbed specific binding substances which specifically binds with substances derived from living organism that are labeled by at least one of labeling substances including radioactive labeling substances, fluorescent substances and chemiluminescent labeling substances.

3. A biochemical analysis unit as described in claim 2, wherein said specific binding substances and said substances derived from living organism are bound through one of hybridization, antigen-antibody reaction and receptor-ligand.

4. A biochemical analysis unit as described in claim 1, wherein said plate member is fixed to said absorptive membrane through an adhesive agent.

5. A biochemical analysis unit as described in claim 3, wherein said plate member decreases a density of said radioactive ray and the light less than 1/5 when said radioactive ray and the light passes in said plate member at a length corresponding to a distance between the nearest two of said through-holes.

6. A biochemical analysis unit as described in claim 5, wherein said plate member decreases a density of said radioactive ray and the light less than 1/10 when said radioactive ray and the light passes in said plate member at a length corresponding to a distance between the nearest two of said through-holes.

7. A biochemical analysis unit as described in claim 6 wherein said plate member is formed of at least one of metallic materials, ceramic materials and plastic materials.

8. A biochemical analysis unit as described in claim 7, wherein said plate member is formed of said plastic materials containing particles of oxides of metals.

9. A biochemical analysis unit as described in claims 8, wherein said absorptive regions and said plate member form a flat surface.

10. A biochemical analysis unit as described in claim 8, wherein said absorptive regions are retracted from a face of said plate member.

11. A biochemical analysis unit as described in claim 3, wherein the number of said absorptive regions is more than 10.

12. A biochemical analysis unit as described in claim 11, wherein the number of said absorptive regions is more than 1000.

13. A biochemical analysis unit as described in claim 12, wherein the number of said absorptive regions is more than 10000.

14. A biochemical analysis unit as described in claim 3, wherein each of said absorptive regions has a size less than 5 mm².

15. A biochemical analysis unit as described in claim 14, wherein each of said absorptive regions has a size less than 1 mm².

16. A biochemical analysis unit as described in claim 15, wherein each of said absorptive regions has a size less than 0.1 mm².

17. A biochemical analysis unit as described in claim 3, wherein the averaged density of the number of said absorptive regions is more than 10/cm².

18. A biochemical analysis unit as described in claim 17, wherein the averaged density of the number of said absorptive regions is more than 1000/cm².

19. A biochemical analysis unit as described in claim 18, wherein the averaged density of the number of said absorptive regions is more than 10000/cm².

20. A biochemical analysis unit as described in claim 3, wherein said plural absorptive regions are arranged in a pattern.

21. A biochemical analysis unit as described in claim 3, wherein said absorptive material is a porous material.

22. A biochemical analysis unit as described in claim 21, wherein said porous material is a carbon porous material or may be used for membrane filter.

23. A biochemical analysis unit as described in claim 3, wherein said absorptive material contains a fiber material.