×

Method and system for measuring a molecular array background signal from a continuous background region of specified size

  • US 7,221,785 B2
  • Filed: 05/21/2002
  • Issued: 05/22/2007
  • Est. Priority Date: 05/21/2002
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for determining a background signal intensity for a particular feature from a representation of scanned intensity values of a molecular array comprising data points and containing discrete features at specified locations, each feature having a region of interest containing a number of data points, the method comprising:

  • specifying a size for a background region corresponding to the feature;

    selecting as background data points those data points within the specified background region;

    filtering the selected data points to remove all data points that lie within specified locations of features and at least one region of interest of features in the region of specified size for the background region; and

    determining the background signal intensity for the particular feature from the filtered, selected data points, wherein background-subtraction of data points from the particular feature using the determined background signal intensity facilitates calculation of accurate and reproducible log ratios between at least one of multi-channel and inter-array data.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×