System, method, and product for dynamic noise reduction in scanning of biological material
First Claim
1. A method for analyzing molecules, comprising the steps of:
- (1) directing at least one filtered excitation beam from at least one excitation source to a first substrate having a control;
(2) determining one or more excitation values which related to the at least one filtered-excitation beam directed to the control;
(3) detecting an emission signal having one or more emission values;
(4) adjusting the at least one excitation source to obtain an excitation reference value when the one or more emission values match a standard value from the control;
(5) scanning a plurality of pixel locations on a second substrate having a plurality of probe locations using the excitation reference value to generate a normalization factor;
(6) adjusting at least one of the one or more emission values based, at least in part, on the normalization factor; and
(7) generating one of either a normalized emission signal and a normalized emission signal data in response to said adjusting of said at least one of the one or more emission values.
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Abstract
Systems and methods are described for processing an emission signal, such as a fluorescent signal, to compensate for noise in an excitation beam, such as a laser beam. As one example, a scanning system is described that includes an excitation signal generator that provides an excitation signal having one or more representative excitation values representative of an excitation beam; an excitation reference provider that provides at least one excitation reference value; a normalization factor generator that compares the excitation reference value to at least one representative excitation value, thereby generating a normalization factor; and a comparison processor that adjusts at least one emission value corresponding to the at least one representative excitation value based, at least in part, on the normalization factor.
58 Citations
31 Claims
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1. A method for analyzing molecules, comprising the steps of:
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(1) directing at least one filtered excitation beam from at least one excitation source to a first substrate having a control; (2) determining one or more excitation values which related to the at least one filtered-excitation beam directed to the control; (3) detecting an emission signal having one or more emission values; (4) adjusting the at least one excitation source to obtain an excitation reference value when the one or more emission values match a standard value from the control; (5) scanning a plurality of pixel locations on a second substrate having a plurality of probe locations using the excitation reference value to generate a normalization factor; (6) adjusting at least one of the one or more emission values based, at least in part, on the normalization factor; and (7) generating one of either a normalized emission signal and a normalized emission signal data in response to said adjusting of said at least one of the one or more emission values. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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2. A method comprising the steps of:
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(1) scanning a plurality of pixel locations on a substrate having a plurality of probe locations to determine an average excitation value; (2) updating an excitation reference value from the average excitation value; (3) directing an excitation beam to the plurality of pixel locations on the substrate; (4) determining one or more representative excitation values, each related to a value of the excitation beam as directed to at least one of the plurality of pixel locations; (5) detecting an emission signal having one or more emission values; (6) correlating each of the one or more emission values with each of the one or more representative excitation values; (7) comparing the excitation reference value to at least one of the one or more representative excitation values, thereby generating a normalization factor; (8) adjusting at least one of the one or more emission values based, at least in part, on the normalization factor; and (9) generating one of either a normalized emission signal and a normalized emission signal data in response to said adjusting of said at least one of the one or more emission values. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification