Peptide nucleic acid conjugates

• US 7,223,833 B1
• Filed: 10/06/1994
• Issued: 05/29/2007
• Est. Priority Date: 05/24/1991
• Status: Expired due to Term

• Current Assignee: Ionis Pharmaceuticals, Inc., Peter E. Nielsen
• Original Assignee: ISIS Pharmaceuticals Incorporated (Ionis Pharmaceuticals, Inc.), Peter E. Nielsen
• Inventors: Manoharan, Muthiah, Kiely, John, Lohse, Jesper, Nielsen, Peter, Egholm, Michael, Sonnechsen, Soren Holst, Griffith, Michael, Sprankle, Kelly, Buchardt, Ole
• Primary Examiner(s): BORIN, MICHAEL L
• Application Number:
  US08/319,411
• Time in Patent Office: 4,618 Days
• Field of Search: 435/6, 435/7.2, 435/890, 436/501, 530/300, 530/350, 530/323, 536/22.1, 536/23.1, 536 2431- 2433, 536/25.3, 514/2, 514/44, 935/77, 935/78
• US Class Current: 530/300
• CPC Class Codes: C07K 14/003 Peptide-nucleic acids (PNAs)
• CPC Class Codes: C12Q 1/6832 Enhancement of hybridisation reaction
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid

Abstract

A novel class of peptide nucleic acids are described which include a conjugate attached thereto. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.

Claims

1. A peptide nucleic acid of the formula:

2. The peptide nucleic acid of claim 1 wherein R⁸, R⁹, R¹⁰ and R¹¹ independently are hydrogen, alkyl, a peptide, a protein, a carbohydrate, a nucleoside, a nucleotide, a nucleotide diphosphate, a nucleotide triphosphate, an oligonucleotide, or an oligonucleoside.

3. The peptide nucleic acid of claim 1 wherein R⁸, R⁹, R¹⁰ and R¹¹ independently are a nucleoside, a nucleotide, an oligonucleotide, or an oligonucleoside.