Apparatus and method for adjustment of ion separation resolution in FAIMS

• US 7,227,132 B2
• Filed: 11/23/2005
• Issued: 06/05/2007
• Est. Priority Date: 11/24/2004
• Status: Expired due to Fees

• Current Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Guevremont, legally incapacitated, Guervremont, legal representative, Kapron, James T.
• Primary Examiner(s): NGUYEN, KIET TUAN
• Application Number:
  US11/285,153
• Publication Number:
  US 20060151693A1
• Time in Patent Office: 559 Days
• Field of Search: 250/287,286,281,282
• US Class Current: 250/287
• CPC Class Codes: G01N 27/624 Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS]

Abstract

An apparatus for controllably varying specificity of a FAIMS-based ion separation includes a first electrode surface portion defining an ion inlet within a portion thereof and a second electrode surface portion defining an ion outlet within a portion thereof. The second electrode surface portion is spaced-apart from the first electrode surface portion along a first direction and disposed in a facing relationship relative to the first electrode surface portion so as to define a FAIMS analyzer region therebetween. An intermediate electrode is disposed between the first electrode surface portion and the second electrode surface portion, the intermediate electrode for defining a transition point of an average ion flow path between the ion inlet and the ion outlet. An actuator is provided for controllably moving the intermediate electrode relative to each of the first electrode surface portion and the second electrode surface portion, for translating the transition point so as to controllably vary the length of the average ion flow path.

Claims

1. An apparatus for controllably varying specificity of a FAIMS-based ion separation, comprising:

a first electrode surface portion defining an ion inlet within a portion thereof;

a second electrode surface portion defining an ion outlet within a portion thereof, the second electrode surface portion spaced-apart from the first electrode surface portion along a first direction and disposed in a facing relationship relative to the first electrode surface portion so as to define a FAIMS analyzer region therebetween;

an intermediate electrode disposed between the first electrode surface portion and the second electrode surface portion, the intermediate electrode for defining a transition point of an average ion flow path between the ion inlet and the ion outlet; and

,

an actuator for controllably moving the intermediate electrode relative to each of the first electrode surface portion and the second electrode surface portion, for translating the transition point so as to controllably vary the length of the average ion flow path.

2. An apparatus according to claim 1, wherein the actuator is for controllably moving the intermediate electrode along a second direction that is normal to the first direction.

3. An apparatus according to claim 1, wherein the actuator is for supporting controllable movement of the intermediate electrode to a position in which a length of the FAIMS analyzer region is approximately zero.

4. An apparatus according to claim 1, wherein the actuator comprises a motor coupled to the intermediate electrode via a linkage member.

5. An apparatus according to claim 1, wherein the actuator comprises a manually operable mechanism coupled to the intermediate electrode.

6. An apparatus according to claim 1, wherein the first electrode surface portion and the second electrode surface portion define surface portions of a same, formed electrode.

7. An apparatus according to claim 1, wherein the first electrode surface portion defines a surface portion of a first electrode and wherein the second electrode surface portion defines a surface portion of a second electrode.

8. An apparatus according to claim 7, wherein the first electrode, the second electrode and the intermediate electrode each comprise a flat electrode body.

9. An apparatus according to claim 8, comprising a temperature controller for controllably varying a temperature of at least one of the first electrode, the second electrode and the intermediate electrode.

10. An apparatus according to claim 8, wherein the intermediate electrode comprises a third electrode surface portion and a fourth electrode surface portion along opposite sides thereof, and comprising an aperture defined within a portion of the intermediate electrode and extending between the third electrode surface portion and the fourth electrode surface portion, the aperture defining the transition point.

11. An apparatus according to claim 10, wherein a periphery of the aperture defines a curved connecting surface between the third electrode surface portion and the fourth electrode surface portion.

12. An apparatus according to claim 8, comprising a curved terminus along one end of the intermediate electrode, the curved terminus disposed between the first electrode surface portion and the second electrode surface portion when in an assembled condition, wherein the transition point is defined adjacent the curved terminus.

13. An apparatus according to claim 12, comprising a second intermediate electrode including a concave surface facing the curved terminus, the concave surface extending between the first electrode surface portion and the second electrode surface portion.

14. An apparatus according to claim 13, wherein the actuator is for moving synchronously the intermediate electrode and the second intermediate electrode, so as to maintain a same spacing from the curved terminus to the concave surface.

15. An apparatus according to claim 7, wherein the first electrode, the second electrode and the intermediate electrode each comprise a curved electrode body.

16. An apparatus according to claim 15, wherein the intermediate electrode comprises a third electrode surface portion and a fourth electrode surface portion disposed along opposite sides thereof.

17. An apparatus according to claim 16, comprising an aperture defined within a portion of the intermediate electrode and extending between the third electrode surface portion and the fourth electrode surface portion, the aperture defining the transition point.

18. An apparatus according to claim 17, wherein a periphery of the aperture defines a curved connecting surface between the third electrode surface portion and the fourth electrode surface portion.

19. An apparatus according to claim 17, wherein the first electrode surface portion and the third electrode surface portion are curved transversely to the second direction such that a distance between the first electrode surface portion and the third electrode surface portion is approximately uniform, and the second electrode surface portion and the fourth electrode surface portion are curved transversely to the second direction such that a distance between the second electrode surface portion and the fourth electrode surface portion is approximately uniform.

20. An apparatus according to claim 19, wherein a thickness of the intermediate electrode between the third electrode surface portion and the fourth electrode surface portion is greater near an edge portion of the intermediate electrode than near a central portion of the intermediate electrode, the central portion adjacent the aperture.

21. An apparatus according to claim 19, wherein a thickness of the intermediate electrode between the third electrode surface portion and the fourth electrode surface portion is approximately uniform between opposite edge portions of the intermediate electrode.

22. A method for controllably varying specificity of a FAIMS-based ion separation, comprising:

providing a FAIMS analyzer region defined by a space between first and second spaced-apart electrode surfaces, the FAIMS analyzer region comprising an ion inlet end and an ion outlet end;

providing an intermediate electrode between the first and second electrode surfaces, the intermediate electrode controllably movable relative to the first and second electrode surfaces for defining a transition point along an average ion flow path between the ion inlet end and the ion outlet end, the transition point for changing a direction of the average ion flow path;

providing a first flow of ions at the ion inlet end of the FAIMS analyzer region;

selectively transmitting a subset of the first flow of ions along the average ion flow path through the FAIMS analyzer region;

moving the intermediate electrode relative to the first and second electrode surfaces, so as to change the length of the average ion flow path for providing a second average ion flow path length between the ion inlet end and the ion outlet end; and

,

transmitting ions of a second flow of ions along the second average ion flow path length.

23. A method according to claim 22, wherein an ion composition of the first flow of ions is substantially the same as an ion composition of the second flow of ions.

24. A method according to claim 23, wherein transmitting ions of a second flow of ions comprises selectively transmitting a subset of the second flow of ions, an ion composition of the subset of the second flow of ions being different than an ion composition of the subset of the first flow of ions.

25. A method according to claim 23, wherein transmitting ions of the second flow of ions comprises transmitting a set of ions having an ion composition substantially the same as the ion composition of the second flow of ions.

26. A method for controllably varying specificity of a FAIMS-based ion separation, comprising:

providing a FAIMS analyzer region having an ion inlet for introducing a flow of ions thereto and having an ion outlet for extracting a subset of the flow of ions that is selectively transmitted along an average ion flow path between the ion inlet and the ion outlet;

introducing a first flow of ions into the FAIMS analyzer region via the ion inlet;

selectively transmitting a subset of the first flow of ions along the average ion flow path;

retaining the ion inlet and the ion outlet in fixed positions one relative to the other;

varying the length of the average ion flow path so as to provide an average ion flow path of a different length through the FAIMS analyzer region; and

,

transmitting ions of a second flow of ions along the average ion flow path of a different length.

27. A method according to claim 26, wherein an ion composition of the first flow of ions is substantially the same as an ion composition of the second flow of ions.

28. A method according to claim 27, wherein transmitting ions of the second flow of ions comprises selectively transmitting a subset the second flow of ions, an ion composition of the subset of the second flow of ions being different than an ion composition of the subset of the first flow of ions.

29. A method according to claim 27, wherein transmitting ions of the second flow of ions comprises transmitting a set of ions having an ion composition substantially the same as the ion composition of the second flow of ions.

30. A method according to claim 26, wherein providing the FAIMS analyzer region comprises providing a space between a first electrode surface and a second electrode surface, the first electrode surface disposed in a parallel facing arrangement relative to the second electrode surface and spaced apart therefrom along a first direction, the first electrode surface defining the ion inlet and the second electrode surface defining the ion outlet.

31. A method according to claim 26, wherein a transition point is defined approximately half way along the average ion flow path between the ion inlet and the ion outlet, the transition point for reversing a velocity component of ion flow in a plane normal to the first direction, and wherein varying the length of the average ion flow path comprises translating the transition point along a second direction parallel to the plane.

32. A method according to claim 31, wherein the transition point is defined by an electrode body intermediate a first electrode surface and a second electrode surface, and wherein translating the transition point comprises relatively translating the electrode body relative to both the first electrode surface and the second electrode surface.