Device for dispensing accurately-controlled small doses of liquid

• US 7,229,594 B2
• Filed: 04/03/2001
• Issued: 06/12/2007
• Est. Priority Date: 04/03/2000
• Status: Expired due to Fees

• Current Assignee: Parabol Technologies SA
• Original Assignee: Parabol Technologies SA
• Inventors: Renaud, Philippe, Donzel, Alain
• Primary Examiner(s): Gordon; Brian R.
• Application Number:
  US10/240,031
• Publication Number:
  US 20030099577A1
• Time in Patent Office: 2,261 Days
• Field of Search: 422/99, 422/93, 422/100, 422/103, 436/180, 436/174, 436/53, 222/321.1, 222/335, 222/372, 222/399, 222/420, 222/400.5, 222/402.16
• US Class Current: 422/521
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 2400/065 sliding valves
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0265 using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The invention provides a device for dispensing accurately-controlled small quantities of at least one liquid, comprising a liquid supply (L); a gas supply (G), arranged to selectively supply a gas pressure; and a capillary duct (20) adapted to be filled with liquid to be dispensed, and to eject the liquid. The device has a filling configuration wherein liquid from the supply is in contact with an end (20a) of the capillary duct to fill the capillary duct with liquid; and a liquid separation and ejection configuration, in which liquid remaining in the liquid supply is separated from liquid that fills the capillary duct to form a discrete quantity (V) of liquid filling the capillary duct (20) out of contact with the remaining liquid of the liquid supply. The capillary duct, filled with this discrete quantity (V) of liquid then has one end in contact with the gas supply and another end in contact with the atmosphere. The discrete quantity of liquid (V), forming an exact dose of liquid to be dispensed, is ejectable from the second end (20b) of the capillary duct by the application of gas pressure.

Claims

1. A device for dispensing small quantities of at least one liquid, comprising:

a reservoir (32;

61) that constitutes a supply of liquid (L) to be dispensed;

a gas supply duct (10;

15;

32;

62) connectable to a gas supply (G), arranged to selectively supply a gas pressure;

a capillary duct (20) having open first and second ends (20a, 20b), the capillary duct being adapted to be filled with liquid (L) to be dispensed, and to eject said liquid;

a wall that forms a bottom wall of the reservoir (32;

61), the capillary duct (20) extending through the thickness of said wall whereby the reservoir is integrated with the liquid-dispensing capillary duct; and

a movable member (50;

15,20,42) movably mounted in the device above said bottom wall of the reservoir (32;

61) between a first position defining a filling configuration and a second position defining a liquid separation and ejection configuration, wherein;

(a) in the filling configuration the movable member (50;

15,20,42) allows access of liquid in the reservoir to the top part of the wall into which the capillary duct (20) opens whereby liquid from the reservoir (32;

61) is in contact with an end (20a) of the capillary duct (20) to fill the capillary duct with liquid; and

(b) in the liquid separation and ejection configuration;

the movable member (50;

15,20,42) blocks access of liquid in the reservoir (32;

61) to a top part of the wall into which the capillary (20) duct opens, so liquid remaining in the reservoir (32;

61) is separated from liquid that fills the capillary_duct (20) to form a discrete quantity (V) of liquid filling the capillary duct out of contact with the remaining liquid (L) of the reservoir;

the capillary_duct, filled with said discrete quantity (V) of liquid has its first end (20a) in contact with the gas supply and its second end (20b) directly or indirectly in contact with the, atmosphere; and

said discrete quantity (V) of liquid is ejectable from the second end (20b) of the capillary duct by the application of gas pressure to the first end (20a) of the capillary duct when the gas supply duct is connected to said ˜

as supply (G),

the integrated reservoir (32;

61) being dimensioned to contain a quantity of liquid (L) that can be dispensed as multiple discrete quantities (V), a diameter or cross-dimension of the integrated reservoir (32;

61) being greater than the diameter of the capillary duct (20) which extends through the thickness of said wail; and

the device being so arranged that the ejected quantities each correspond precisely to the discrete quantity (V) that fills the capillary duct (20) in the liquid separation and ejection configuration.

2. The device of claim 1, wherein the first end (20a) of the capillary duct branches from a liquid and gas supply duct which is of greater diameter than the capillary duct, the liquid and gas supply duct having a liquid supply end connected to the liquid supply (L) and a gas supply end (10) connectable to the gas supply (G), wherein said movable member is a piston (50) slidably mounted in the liquid and gas supply duct, the piston separating the liquid and gas supply, the piston being movable between a first position defining said filling configuration and a second position defining said separation and ejection configuration.

3. The device of claim 2, which comprises a dispensing nozzle that incorporates the capillary duct (20) and the liquid and gas supply duct.

4. The device of claim 2, comprising a plurality of capillary ducts (20) branched from the liquid and gas supply duct, wherein in the filling configuration liquid from the liquid supply (L) is in contact with an end of each capillary duct (20) to fill each capillary duct with liquid, and in the liquid separation and ejection configuration:

liquid remaining in the liquid supply (L) is separated from liquid that fills each capillary duct (20) to form a discrete quantity (V) of liquid filling each capillary duct out of contact with the remaining liquid of the liquid supply;

each capillary duct (20), filled with its discrete quantity (V) of liquid, has its first end (20a) in contact with the gas supply duct (10) and its second end (20b) in contact with the atmosphere; and

said discrete quantities (V) of liquid in the capillary ducts (20) are ejectable simultaneously from the second ends (20b) of the capillary ducts by the application of gas pressure to the first ends (20a) of the capillary ducts.

5. The device of claim 2, wherein the liquid and gas supply duct has at least two liquid supply sections connected to respective liquid supplies (L1, L2) and branched together to a gas supply section from which the capillary duct (20) is branched, said liquid supplies being arranged to control liquid in the liquid supply sections to bring liquid from a selected liquid supply section over the first end (20a) of the capillary duct for the ejection of a discrete quantity of the selected liquid.

6. The device of claim 1, wherein the capillary duct (20) further comprises a movable part of the capillary duct that is carried by the gas supply duct (15), the gas supply duct being movably mounted relative to the liquid reservoir (L) between a position in which the movable part of the capillary duct is aligned with but spaced from the capillary duct in said bottom wall for intaking liquid and a position in which the movable part of the capillary duct is aligned with and against the capillary duct in the bottom wall for subsequently ejecting liquid.

7. The device of claim 6, wherein the movable part of the capillary duct (20) is mounted at an end of a gas supply duct (15) of greater diameter, with the first end (20a) of the movable part of the capillary duct leading inside the gas supply duct (15) and the second end (20b) of the movable part of the capillary duct protruding from the gas supply duct, said liquid supply comprising a reservoir (32) of liquid (L), the gas supply duct (15) being movably mounted in the reservoir and movable between a first position defining said filling configuration wherein the second end (20b) of the capillary duct is in communication with liquid (L) in the reservoir, and a second position defining said separation and ejection configuration, wherein in its second position the movable part of the capillary duct (20) abuts by its second end (20b) against a wall of the reservoir (32), the reservoir having a capillary opening (36) in extension of the movable part of the capillary duct (20) such that in said filling configuration a discrete quantity of liquid to be dispensed is contained in the movable part of the capillary duct (20) extended by said capillary opening (36).

8. A device according to claim 1, further comprising a support (42) which has a bottom wall including the capillary duct (20), wherein the liquid supply reservoir (61) and the gas supply duct (62) are contained in said movable member (60) which is arranged for displacement relative to the support (42) to selectively bring the gas supply duct (62) into or out of alignment with the capillary duct (20).

9. The device of claim 8, wherein the movable member (60) is generally cylindrical and is rotatably mounted relative to the support (42);

the gas supply duct (62) is an eccentric bore parallel to the axis of rotation of the member (60), and the support (42) has a bottom with the capillary bore (20) with which the gas duct (62) can be brought into or out of alignment by rotation of the member (60).

10. An assembly comprising an array of the devices as claimed in claim 9, which devices are actuable in rotation individually or together.

11. The device of claim 8 wherein the movable member is a slide plate (60) that is slidably mounted between opposing stationary plates (65, 66) respectively containing gas supply openings (62) and capillary ducts (20), the slide plate (60) containing a series of liquid reservoirs (L1, L2, . . . ) and gas supply ducts (62).

12. The device of claim 1, further comprising a gas supply (G) connected to the gas supply duct (10;

15;

32;

62) and arranged to supply a gas pressure to the gas supply duct.