Method for measuring blood urea level by reverse iontophoresis

• US 7,231,242 B2
• Filed: 04/08/2003
• Issued: 06/12/2007
• Est. Priority Date: 04/08/2002
• Status: Expired due to Fees

• Current Assignee: Metin Denli, Rusen Dundaroz, Sibel Ilbasmis, Tuncer Degim, Zelihagul Degim
• Original Assignee: Metin Denli, Rusen Dundaroz, Sibel Ilbasmis, Tuncer Degim, Zelihagul Degim
• Inventors: Degim, Zelihagul, Dundaroz, Rusen, Ilbasmis, Sibel, Denli, Metin, Degim, Tuncer
• Primary Examiner(s): Marmor; Charles
• Assistant Examiner(s): Natnithithadha; Navin
• Application Number:
  US10/510,779
• Publication Number:
  US 20050273045A1
• Time in Patent Office: 1,526 Days
• Field of Search: 600/306, 600/309, 600345-397
• US Class Current: 600/361
• CPC Class Codes: A61B 5/145 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissuemeasuring of blood pressure or blood flow A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: A61N 1/30 Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis

Abstract

A method for measuring and determining urea level in human blood by reverse iontophoresis comprising the following steps of:

• • placing an anode electrode on skin,
  • placing a container comprising a solution including urease and a cathode electrode on skin,
  • applying electric current to the anode electrode and the cathode electrode so that an electrical circuit is established there between, whereby urea molecules migrate from blood into the container,
  • determining the urea level in the blood by the changes in the pH of the urease solution.

Claims

1. A device for determining from a contact area on a patient'"'"'s skin the urea level in the patient'"'"'s blood, which comprises:

a housing designed to be secured to a patient'"'"'s limb;

an anode;

a container including a cathode, an exterior, a solution including urease, and a membrane operatively interposed between said solution and the exterior of the said container, wherein said anode and said membrane being capable of making contact with the patient'"'"'s skin;

means for applying a potential difference between said anode and said cathode so as to produce reverse iontophoresis to cause urea molecules to migrate to said container;

means for determining the pH of the solution before and after said potential difference has been applied; and

means for measuring the urea level in the patient'"'"'s blood based on the change of the pH of the solution.

2. The device of claim 1, in which said container also contains an isotonic saline solution.

3. The device of claim 1, in which said container contents are in the form of a gel.

4. The device of claim 2, in which said container contents are in the form of a gel.

5. The method of determining from a contact area on the patient'"'"'s skin the urea level in the patient'"'"'s blood which comprises (a) placing on the skin at said contact area an anode and a container containing a cathode and a solution including urease and having a dialysis membrane adapted to be operatively interposed between said solution and said contact area, (b) applying a potential difference between said anode and said cathode so as to produce reverse iontophoresis to cause urea molecules to migrate into said container, and (c) measuring the urea level in the patient'"'"'s blood by determining the change in pH of said urease before and after said potential difference has been applied.

6. The method of claim 5, in which said solution includes an isotonic saline solution.

7. The method of claim 5, in which said solution is in the form of a gel.

8. The method of claim 6, in which said solution is in the form of a gel.

9. In the method of claim 6, using a software controlled microcomputer for determining the urea level in the patient'"'"'s blood.

10. The method of claim 5, in which the anode, the container with its contents, and means for applying said potential difference are embodied into a limb-attachable wristwatch-like device.