Nucleic acid analysis device
First Claim
1. A system for detecting or quantitating one or more target polynucleotide sequences in a liquid sample, comprising:
- a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, (iii) a channel providing a fluid communication to said inlet, and (iv) a plurality of dead-end fluid connections, one dead-end fluid connection between each of said two or more detection chambers and said channel, wherein said two or more detection chambers are each pre-loaded with a primer pair suitable for polymerase chain reaction and a sequence-specific fluorescently labeled oligonucleotide for detecting or quantitating a selected one of the one or more target polynucleotide sequences in the respective detection chamber and adapted to produce a detectable signal upon reacting with the selected one of the one or more target polynucleotide sequences;
a temperature-controller adapted to heat or cool the two or more detection chambers; and
a fluorescence detector configured for optical communication with one or more of the two or more detection chambers, for detecting fluorescence produced therein.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
176 Citations
13 Claims
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1. A system for detecting or quantitating one or more target polynucleotide sequences in a liquid sample, comprising:
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a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, (iii) a channel providing a fluid communication to said inlet, and (iv) a plurality of dead-end fluid connections, one dead-end fluid connection between each of said two or more detection chambers and said channel, wherein said two or more detection chambers are each pre-loaded with a primer pair suitable for polymerase chain reaction and a sequence-specific fluorescently labeled oligonucleotide for detecting or quantitating a selected one of the one or more target polynucleotide sequences in the respective detection chamber and adapted to produce a detectable signal upon reacting with the selected one of the one or more target polynucleotide sequences; a temperature-controller adapted to heat or cool the two or more detection chambers; and a fluorescence detector configured for optical communication with one or more of the two or more detection chambers, for detecting fluorescence produced therein. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A system for detecting or quantitating a one or more target polynucleotide sequences in a liquid sample, comprising:
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a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said two or more detection chambers and said inlet, wherein said two or more detection chambers are each pre-loaded with a primer pair suitable for reacting with a selected one of the one or more target polynucleotide sequences, and a sequence-specific fluorescently labeled oligonucleotide for detecting or quantitating the selected one of the one or more target polynucleotide sequences in the respective detection chamber and adapted to produce a detectable signal upon reacting with the selected one of the one or more target polynucleotide sequences; means for modulating the temperature of the two or more detection chambers to effect thermal cycling of the liquid sample within each detection chamber; and a fluorescence detector configured for optical communication with one or more of the two or more detection chambers, for detecting fluorescence produced therein. - View Dependent Claims (8, 9, 10, 11, 12)
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13. An assembly for detecting or quantitating one or more target polynucleotide sequences in a liquid sample, comprising:
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a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, (iii) a channel providing a fluid communication to said inlet, and (iv) a plurality of dead-end fluid connections, one dead-end fluid connection between each of said two or more detection chambers and said channel, wherein said two or more detection chambers are each pre-loaded with a primer pair suitable for reacting with a selected one of the one or more target polynucleotide sequences, and a sequence-specific fluorescently labeled oligonucleotide for detecting or quantitating the selected one of the one or more target polynucleotide sequences in the respective detection chamber and adapted to produce a detectable signal upon reacting with the selected one of the one or more target polynucleotide sequences; means for modulating the temperature of the two or more detection chambers to effect thermal cycling of the liquid sample within each detection chamber; and a fluorescence detector configured for optical communication with one or more of the two or more detection chambers, for detecting fluorescence produced therein.
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Specification