Immunogen adherence inhibitor directed to lactic acid producing organisms and method of making and using it

• US 7,238,351 B2
• Filed: 09/08/2003
• Issued: 07/03/2007
• Est. Priority Date: 07/15/1999
• Status: Expired due to Term

• Current Assignee: Bradley M. Mitteness, Peter Nash
• Original Assignee: Bradley M. Mitteness, Peter Nash
• Inventors: Mitteness, Bradley M., Nash, Peter
• Primary Examiner(s): Chan; Christina
• Assistant Examiner(s): HUYNH, PHUONG N
• Application Number:
  US10/658,491
• Publication Number:
  US 20040043020A1
• Time in Patent Office: 1,394 Days
• Field of Search: 424/130.1, 424/164.1, 424/169.1, 435/69.1, 530/389.5
• US Class Current: 424/130.1
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: C07K 16/02 from eggs
• CPC Class Codes: C07K 16/12 against material from bacteria
• CPC Class Codes: C07K 16/1203 from Gram-negative bacteria
• CPC Class Codes: C07K 16/1275 from Streptococcus (G)
• CPC Class Codes: C07K 2317/23 from birds

Abstract

The method of producing a microbial adherence inhibitor for administration to food animals to control the incidents of acidosis in food animals by preventing the adherence of colony-forming lactic acid producing bacteria in the rumen or intestinal tracts of the food animals. The method includes inoculating female birds in or about their egg laying age with a lactic acid producing bacteria that colonizes in the intestinal tract of the food animal to be treated. Allowing a period of time sufficient to permit the production in the birds of antibody to the lactic acid producing bacteria and harvesting the eggs laid by such birds. The antibody-containing contents of the eggs are then separated by the shells.

Claims

1. A method of production of a microbial adherence inhibitor for administration to food animals to control the incidence of rumen acidosis in food animals by inhibiting the adherence of either Streptococcus bovis or Lactobacillus spp. bacteria in the rumen of said food animals, which method comprises:

a. inoculating female birds, in or about to reach their egg laying age, with either Streptococcus bovis or Lactobacillus spp. bacteria that have been cultured to stimulate adherence antigens for either Streptococcus bovis or Lactobacillus spp.;

b. allowing a period of time sufficient to permit the production in the birds of antibody to to the adherence antigens of either Streptococcus bovis or Lactobacillus spp. bacteria;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells to obtain said microbial adherence inhibitor.

2. The method of claim 1 including:

drying the separated antibody-containing contents of said eggs.

3. The method of claim 2 including:

providing a dry feed carrier material, and drying the separated antibody-containing contents of said eggs by coating said dry carrier material with said separated antibody-containing contents of said eggs.

4. The method of claim 3 wherein:

the dry feed carrier material is selected from the group consisting of soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

5. The method of claim 1 including:

mixing the antibody-containing contents of the eggs with a liquid extender palatable to the food animal.

6. A method of production of a microbial adherence inhibitor for administration to food animals to control the incidence of rumen acidosis in food animals by inhibiting the adherence of Streptococcus bovis bacteria in the rumen of said food animals, said method comprising:

a. inoculating female birds, in or about to reach their egg laying age, with the Streptococcus bovis that have been cultured to stimulate adherence SB antigens from Streptococcus bovis;

b. allowing a period of time to permit the production in the birds and eggs laid by the birds of antibody to SB antigen from Streptococcus bovis;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said harvested eggs from the eggshells to obtain the microbial adherence inhibitor.

7. The method of claim 6 further comprising:

drying said antibody-containing contents of the eggs.

8. The method of claim 6 further comprising providing a dry feed carrier material, and drying the separated antibody-containing contents of said eggs by coating said dry feed carrier material with said separated antibody-containing contents of said eggs.

9. The method of claim 8 wherein:

the dry feed carrier material is selected from the group consisting of soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

10. The method of claim 6 including:

mixing the antibody-containing contents of the eggs with a liquid extender palatable to the food animal.

11. A microbial adherence inhibitor for administration to food animals to control the incidence of rumen acidosis caused by either Streptococcus bovis or lactobacillus spp. bacteria by reducing the ability of the bacteria to multiply, produced by the method of:

a. inoculating female birds, in or about to reach their egg laying age, with either Streptococcus bovis or lactobacillus spp. bacteria that have been cultured to stimulate adherence antigens;

b. allowing a period of time sufficient to permit the production in the birds of antibody to the adherence antigens of either Streptococcus bovis or Lactobacillus spp. bacteria;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells to obtain the microbial adherence inhibitor.

12. The microbial adherence inhibitor of claim 11 wherein the method includes:

drying the antibody-containing contents of the eggs.

13. The microbial adherence inhibitor of claim 11 further comprising

coating a dry feed carrier with said antibody containing contents of said eggs to obtain

a dry feed carrier coated with the antibody-containing contents of the eggs.

14. The microbial adherence inhibitor of claim 13 wherein:

the dry feed carrier material is selected from the group consisting of soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

15. The microbial adherence inhibitor of claim 11 further comprising mixing the antibody-containing contents of the eggs with a liquid extender.

16. The microbial adherence inhibitor of claim 15 wherein:

said liquid extender is either liquid molasses or PBS.

17. A microbial adherence inhibitor for administration to food animals to control the incidence of rumen acidosis caused by Streptococcus bovis bacteria in the rumen of said food animals by reducing the ability of the bacteria to multiply, produced by the method of:

a. inoculating female birds, in or about to reach their egg laying age, with SB antigen from Streptococcus bovis;

b. allowing a period of time sufficient to permit the production in the birds and eggs laid by the birds of antibody to SB antigen from Streptococcus bovis;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells to obtain the microbial adherence inhibitor.

18. The microbial adherence inhibitor of claim 17 wherein said method includes:

drying said antibody-containing contents of said eggs.

19. The microbial adherence inhibitor of claim 17 further comprising coating a dry feed carrier with said antibody containing contents of said eggs to obtain

a dry feed carrier material coated with the antibody-containing contents of said eggs.

20. The microbial adherence inhibitor of claim 19 wherein:

the dry feed carrier material is selected from the group consisting of soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

21. The microbial adherence inhibitor of claim 17 further comprising mixing the antibody containing contents of the eggs with a liquid extender palatable to the food animal.

22. A method for substantially reducing or eliminating

the incidence of rumen acidosis in food animals caused by the presence of lactic acid forming bacteria in the animal by inhibiting the ability of the bacteria to adhere to the rumen of the animal to reduce the ability of the bacteria to multiply, said method comprising:

a. inoculating female birds, in or about to reach their egg laying age, with Streptococcus bovis bacteria whose colonization results in rumen acidosis and the Streptococcus bovis having been cultured to stimulate adherence SB antigens;

b. allowing a period of time sufficient to permit the production in the birds of antibody to Streptococcus bovis adherence antigens;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells;

e. drying said separated antibody-containing contents of said eggs;

f. distributing the resulting dried egg antibody product substantially uniformly through an animal feed or water; and

g. supplying the resulting antibody-containing animal feed or water to food animals to substantially inhibit adherence of Streptococcus bovis bacteria to the rumen, thereby substantially reducing or eliminating the incidence of rumen acidosis in food animal.

23. The method of claim 22 further comprising providing a dry feed carrier material, and drying the separated antibody-containing contents of said eggs by coating said dry carrier material with said separated antibody-containing contents of said eggs.

24. The method of claim 23 wherein:

the dry feed carrier material is selected from the group consisting of soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

25. A method for substantially reducing or eliminating the incidence of rumen acidosis in food animals caused by the presence of either Streptococcus bovis or Lactobacillus spp. bacteria in the animal by inhibiting the ability of the bacteria to adhere to the rumen of the animal to reduce the ability of the bacteria to multiply, said method comprising:

a. inoculating female birds, in or about to reach their egg laying age, with either Streptococcus bovis or Lactobacillus spp. bacteria that have been cultured to stimulate adherence antigens;

b. allowing a period of time sufficient to permit the production in the birds of antibody to either Streptococcus bovis or Lactobacillus spp. adherence antigens;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells;

e. distributing the resulting egg mixture antibody product substantially uniformly through an animal feed or water; and

f. supplying the resulting antibody-containing animal feed or water to food animals to substantially reduce adherence of either Streptococcus bovis or Lactobacillus, spp. bacteria to the rumen thereby substantially reducing or eliminating the incidence of rumen acidosis in food animal.

26. The method of claim 25 further comprising

mixing the antibody-containing contents of said eggs with a liquid extender.

27. The method of claim 26 wherein:

said liquid extender is either liquid molasses or PBS.