Immunoassay method/equipment, biological component measurable toilet, anti-albumin monoclonal antibody, cell strain producing the same, and albumin detection kit

• US 7,241,578 B2
• Filed: 05/20/2003
• Issued: 07/10/2007
• Est. Priority Date: 05/22/2002
• Status: Expired due to Fees

• Current Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Kawamura, Tatsurou, Shigetoh, Nobuyuki, Kamei, Akihito, Yugawa, Keiko
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): GRUN, JAMES LESLIE
• Application Number:
  US10/441,192
• Publication Number:
  US 20030219910A1
• Time in Patent Office: 1,512 Days
• Field of Search: 435/7.1, 435/7.8, 435/70.21, 435/452, 435/337, 436/517, 436/518, 436/536, 436/537, 436/539, 436/548, 436/164, 530/388.25
• US Class Current: 435/7.1
• CPC Class Codes: A61B 10/0038 Devices for taking faeces samples; Faecal examination devices anal receptacles A61F5/451
• CPC Class Codes: A61B 10/007 for taking urine samples measuring urological functions A61B5/20; genital receptacles A61F5/451; urinals for bed-ridden persons A61G9/006
• CPC Class Codes: A61B 5/20 for measuring urological functions restricted to the evaluation of the urinary system A61B5/4375 takes precedence
• CPC Class Codes: A61B 5/415 the glands, e.g. tonsils, adenoids or thymus
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 33/577 involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens; G01N33/53 - G01N33/576 take precedence
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92

Abstract

The immunoassay method of this invention measures the content of a subject substance in a sample. The method includes the steps of: (a) preparing a mixed solution by mixing the sample and an antibody solution including a first monoclonal antibody and a second monoclonal antibody capable of specifically binding to the subject substance; and (b) measuring an optical property of the mixed solution. The first monoclonal antibody is capable of binding to a first epitope of the subject substance, and the second monoclonal antibody is capable of binding to a second epitope of the subject substance different from the first epitope. Each of the first and second epitopes exists singly in the subject substance.

Claims

1. An anti-albumin monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-7938.

2. An anti-albumin monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-7937.

3. An anti-albumin monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-8307.

4. An anti-albumin monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-8308.

5. An anti-albumin monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-8309.

6. A cell strain deposited as Accession Number FERM BP-7938.

7. A cell strain deposited as Accession Number FERM BP-7937.

8. A cell strain deposited as Accession Number FERM BP-8307.

9. A cell strain deposited as Accession Number FERM BP-8308.

10. A cell strain deposited as Accession Number FERM BP-8309.

11. An immunoassay method for measuring a content of human albumin in a sample, comprising the steps of:

(a) providing an antibody solution including a first monoclonal anti-albumin antibody, a second monoclonal anti-albumin antibody and a third monoclonal anti-albumin antibody all capable of specifically binding to different epitopes of human albumin, wherein each of the epitopes bound by the first and the second monoclonal antibodies exists singly in albumin, and wherein

the first monoclonal antibody is a monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-7937;

the second monoclonal antibody is a monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-7938; and

the third monoclonal antibody is a monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-8307;

(b) preparing a mixed solution by mixing the sample and the antibody solution under conditions sufficient for the monoclonal antibodies to specifically bind to albumin in the sample; and

(c) measuring an optical property of the mixed solution, wherein the measured optical property is proportional to the content of human albumin in the sample.

13. The immunoassay method claim 11 or 12 wherein the measured optical property is intensity of scattered light or amount of transmitted light.

12. An immunoassay method for measuring a content of human albumin in a sample, comprising the steps of:

(a) providing an antibody solution including a first monoclonal anti-albumin antibody, a second monoclonal anti-albumin antibody and a third monoclonal anti-albumin antibody all capable of specifically binding to different epitopes of human albumin, wherein each of the epitopes bound by the first and the second monoclonal antibodies exists singly in albumin, and wherein

the first monoclonal antibody is a monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-7938;

the second monoclonal antibody is a monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-8308; and

the third monoclonal antibody is a monoclonal antibody produced from a cell strain deposited as Accession Number FERM BP-8309;

(b) preparing a mixed solution by mixing the sample and the antibody solution under conditions sufficient for the monoclonal antibodies to specifically bind to albumin in the sample; and

(c) measuring an optical property of the mixed solution, wherein the measured optical property is proportional to the content of human albumin in the sample.