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Process for preparing cyanine dye labeling reagents

  • US 7,241,897 B2
  • Filed: 01/21/2004
  • Issued: 07/10/2007
  • Est. Priority Date: 03/12/2002
  • Status: Expired due to Term
First Claim
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1. A process for preparing a cyanine dye labeling reagent, said process comprising the steps of:

  • (a) providing;

    (i) a first intermediate compound comprising;

    embedded image wherein X1 comprises carbon, oxygen, nitrogen or sulfur; and

    (ii) a second intermediate compound comprising;

    embedded image wherein X2 comprises carbon, oxygen, nitrogen or sulfur;

    wherein at least one of R1 through R10 comprises a reactive group capable of forming a carbon-carbon linkage with a target, and(iii) linking reagents suitable for linking said first intermediate compound and said second intermediate compound;

    (b) forming a reaction mixture comprising said first intermediate compound (i), said second intermediate compound (ii), and said linking reagents under conditions to link (i) and (ii) to form embedded image wherein at least one of R1 through R10 comprises a reactive group capable of forming a carbon-carbon linkage with a target, wherein said reactive group comprises an alkene group, an alkyne group, or a metallo-organic compound, and wherein n is an integer of 1, 2 or 3, and wherein X1 and X2 independently comprise carbon, oxygen, nitrogen or sulfur.

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