Method and apparatus for high resolution coherent optical imaging

• US 7,242,833 B2
• Filed: 07/10/2001
• Issued: 07/10/2007
• Est. Priority Date: 07/10/2000
• Status: Expired due to Term

• Current Assignee: University Health Network
• Original Assignee: University Health Network
• Inventors: Wilson, Brian C., Yang, Victor Xiao Dong, Katz, Sharon, Vitkin, I. Alex, Mok, Alvin Ho Kwan, WongKeeSong, Louis M., Gordon, Margaret Leslie
• Primary Examiner(s): Connelly-Cushwa; Michelle
• Application Number:
  US10/311,358
• Publication Number:
  US 20040076390A1
• Time in Patent Office: 2,191 Days
• Field of Search: 385115-119
• US Class Current: 385/117
• CPC Class Codes: A61B 1/00096 Optical elements
• CPC Class Codes: A61B 1/00172 with means for scanning
• CPC Class Codes: A61B 1/05 characterised by the image sensor, e.g. camera, being in the distal end portion
• CPC Class Codes: A61B 5/0066 Optical coherence imaging
• CPC Class Codes: A61B 5/6852 Catheters
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

A method and an apparatus for examining the subsurface microstructure of a sample are provided. Radiation from a plurality of optical radiation sources travels along a first optical path. In the first optical path, a device focuses the optical radiati n from each of the optical sources into a plurality of respective focal points along the first optical path to provide substantially continuous coverage of a selected portion of the first optical path. Then, a sample on the first optical path within the selected length extending into the sample is scanned along said selected portion of the first optical path.

Claims

1. An apparatus for optical examination of a sample, the apparatus comprising:

an optical source means for providing a plurality of separate optical radiation sources;

a first optical path extending from the optical source means;

a focusing means in the first optical path for focusing optical radiation from the optical radiation sources into a plurality of respective focal points located on a surface within the first optical path to provide substantially continuous coverage of a selected portion of the first optical path, wherein, in use, a sample can be located at least partially within said selected portion, thereby permitting simultaneous scanning of a plurality of points within the sample,

wherein the optical source means comprises optical coupling means having one of a plurality of optical fibers and a plurality of optical waveguide wafers, wherein ends of the plurality of optical fibers or the plurality of optical waveguide wafers are stepped relative to one another along the first optical path and wherein optical radiation from each optical fiber or each optical waveguide wafer is focused to a different focal point on the surface of the first optical path.

2. An apparatus as claimed in claim 1, wherein the optical source means further comprises a primary optical source and wherein the optical coupling means connects the primary optical source to the optical radiation sources.

3. An apparatus as claimed in claim 2, which further includes a plurality of optical couplers between the primary optical source and the optical fibers, an optical delay generator coupled to the optical couplers and detector means coupled to the optical couplers, wherein the optical couplers transmit a portion of the radiation from the primary optical source along the first optical path and a portion of the radiation from the primary optical source to the optical delay generator, the optical delay generator providing a second optical path, wherein an interference effect occurs between radiation returned along the first and second optical paths to the optical couplers and the optical couplers transmit the radiation returned along the first and second optical paths to the detector means.

4. An apparatus as claimed in claim 3, which includes a plurality of primary optical sources and a plurality of tree couplers, each tree coupler being associated with one primary optical source and coupling said one primary optical source to at least one of the optical couplers.

5. An apparatus as claimed in claim 4, which includes a plurality of optical circulators, placed between the optical couplers and the detectors for providing salvaged optical radiation to the detectors.

6. An apparatus as claimed in claim 3, wherein a plurality of optical fibers couple the optical couplers to the optical delay generator, the optical delay generator having a grating and a scanning mirror, the scanning mirror having an axis, wherein the grating separates optical radiation from each optical fiber into spectral components linearly oriented on the scanning mirror, wherein the midpoint of said spectral components is offset from the axis of the scanning mirror by a distance x_d to phase modulate said spectral components.

7. An apparatus as claimed in claim 3, which includes a first plurality of optical fibers for coupling each optical coupler to a phase modulator and a second plurality of optical fibers for coupling each phase modulator to the optical delay generator, the optical delay generator having a grating and a scanning mirror, the scanning mirror having an axis, wherein for each optical fiber, the phase modulator phase modulates the optical radiation and said grating separates said phase modulated optical radiation into spectral components which are linearly oriented on said scanning mirror, the midpoint of said spectral components being centered on the axis of said scanning mirror and wherein the spectral components from each optical fiber is spaced apart from the spectral components of the optical radiation from the other optical fibers.

8. An apparatus as claimed in claim 3, which includes a plurality of optical fibers coupling each optical coupler to a tree coupler for combining optical radiation from each of the optical fibers into a first optical fiber, said first optical fiber connected to a phase modulator for phase modulating said combined optical radiation and including a second optical fiber for coupling said phase modulator and said optical delay generator.

9. An apparatus as claimed in claim 1, wherein the optical source means further comprises a plurality of optical sources (50), and wherein the optical coupling means connects the plurality of optical sources (50) to the optical radiation sources and further includes a plurality of optical couplers (54) adapted to adjust the intensity of the optical radiation provided by each of the plurality of optical fibers (10) or each of the plurality of optical waveguide wafers for facilitating deep or shallow scanning.

10. An apparatus as claimed in claim 1, which includes a rotatable mirror in the first optical path, for deflecting radiation from the optical radiation sources to permit rotational movement of the surface.

11. An apparatus as claimed in claim 10, including a micromachined electro-mechanical system coupled to the mirror for rotating the mirror.

12. An apparatus as claimed in claim 10, wherein the apparatus is configured as an endoscope for internal examination of a body, wherein the first optical path, the focusing means and the mirror are provided in the endoscope, and wherein the endoscope includes at least one of:

at least one channel for white light illumination;

a channel for white light endoscope forward viewing;

a channel for one of an air nozzle and a water nozzle; and

a suction/biopsy channel.

13. An apparatus as claimed in claim 12, wherein the endoscope is adapted to perform radial, translational or helical scanning.

14. An apparatus as claimed in claim 13, wherein the endoscope further includes a micromachined electro-mechanical system as a drive mechanism.

15. An apparatus as claimed in claim 1, which includes a mirror in the first optical path and wherein the optical source means, the focusing means and the mirror, in combination, may be linearly translated to permit linear movement of the surface.

16. An apparatus as claimed in claim 15, including a micromachined electro-mechanical system for linearly translating the optical source means, the focusing means and the mirror.

17. An apparatus as claimed in claim 1, which includes a rotatable mirror in the first optical path and wherein the optical source means, the focusing means and the rotatable mirror, in combination, may be linearly translated to permit helical movement of the surface.

18. A method for optical examination of a sample, the method comprising:

(a) providing radiation from a plurality of separate optical radiation sources, along a first optical path;

(b) providing focusing means in the first optical path;

(c) focusing the optical radiation from the optical sources into a plurality of respective focal points along a surface within the first optical path to provide substantially continuous coverage of a selected portion of the first optical path;

(d) providing a sample located at least partially within the first optical path;

(e) simultaneously scanning a plurality of points within the sample, and

(f) providing an optical coupling means for the plurality of separate optical radiation sources wherein the optical coupling means have one of a plurality of optical fibers or a plurality of optical waveguide wafers, wherein the method further comprises stepping the ends of the plurality of optical fibers or the plurality of optical waveguide wafers relative to one another in a common plane along the first optical path for focusing optical radiation from each optical fiber or each optical waveguide wafer through a common lens to a different focal point on the surface of the first optical path.

19. A method as claimed in claim 18, which includes providing radiation from a primary optical source and transmitting the radiation along the plurality of optical fibers or the plurality of optical waveguide wafers to the plurality of separate optical radiation sources.

20. A method as claimed in claim 18, wherein the method further includes providing a plurality of optical sources, connecting the plurality of optical sources to the optical radiation sources and using a plurality of optical couplers to adjust the intensity of the optical radiation provided by each of the plurality of optical fibers or each of the plurality of optical waveguide wafers for facilitating deep or shallow scanning.

21. A method as claimed in claim 18, which includes:

(a) providing a rotatable mirror in the first optical path;

(b) deflecting the first optical path;

(c) causing the plurality of focal points to be located on a surface;

(d) performing an axial scan;

(e) performing one of;

rotating the mirror to move the surface;

linearly translating, in combination, the focusing means, the plurality of optical radiation sources and the mirror to move the surface; and

, simultaneously rotating the mirror and linearly translating, in combination, the focusing means, the plurality of optical radiation sources and the mirror to move the surface; and

,

(f) repeating step (d) at least two times and performing step (e) between each repetition.

22. A method as claimed in claim 21, which includes supplying radiation from the primary optical source through a plurality of couplers to the optical fibers, providing an optical delay generator connected to the optical couplers and providing a second optical path, permitting radiation to be transmitted back along the first and second optical paths to the couplers, for forming interference, and transmitting radiation received from the first and second optical paths at the optical couplers to detection means for detection of the interference pattern.

23. A method as claimed in claim 22, which includes providing a plurality of primary optical sources and, for each primary optical source, a respective tree coupler, and coupling each primary optical source through said respective tree coupler to each of the optical couplers.

24. A method as claimed in claim 23, which includes providing the apparatus as an endoscope adapted for examining an internal cavity of the body, including at least one of:

at least one channel for white light illumination, a white light endoscope forward viewing channel, a suction/biopsy channel and a channel for one of an air nozzle and a water nozzle.

25. A method as claimed in claim 22, which includes providing optical circulator means between the optical couplers and the detection means for providing salvaged optical radiation to the detection means.

26. A method as claimed in claim 18, wherein there is a change in refractive index between a medium containing the optical radiation sources and the sample, wherein for each focal point, a distance mismatch due to the change in refractive index between the coherence gate of each optical radiation source and the focal point in the sample is obtained according to the steps of:

(a) scanning optical radiation from the plurality of optical radiation sources in said first optical path such that the focal points of the optical radiation sources are aligned along a path extending from the medium containing the optical radiation sources into the sample;

(b) detecting the reflected optical radiation for each focal point; and

,

(c) locating the focal point for which there is a large change in reflected optical radiation compared to neighboring focal points,

wherein, the focal point located in step (c) indicates the location of the interface between the sample and the medium containing the optical radiation sources.

27. A method as claimed in claim 26, wherein the method further comprises repeating steps (a) to (c) for each axial scan to obtain proper spatial resolution for the selected scan zone.