Multiaperture sample positioning and analysis system
First Claim
Patent Images
1. A method for analyzing a plurality of membranous samples, such as cells, vesicles, and cellular organdies, comprising:
- selecting a measuring arrangement, comprising (i) a holder, and (ii) a plurality of measurement sites disposed in the holder, each measurement site having a carrier that separates from one another two fluid compartments, the carrier containing an aperture that connects the two compartments, wherein each carrier includes a binding surface adjacent the aperture that is adapted to seal a sample across the aperture, wherein each compartment is in electrical contact with at least one electrode, and wherein the diameter of each aperture immediately adjacent the binding surface is between about 0.3 μ
m and about 7 μ
m;
providing a suspension of membranous samples to one of the fluid compartments of at least two measurement sites;
sealing a first sample from the suspension across a first aperture in the measuring arrangement;
measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the first sample, after the first sample is sealed across the first aperture;
sealing a second sample from the suspension across a second aperture in the measuring arrangement; and
measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the second sample, after the second sample is sealed across the second aperture.
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Abstract
Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.
178 Citations
67 Claims
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1. A method for analyzing a plurality of membranous samples, such as cells, vesicles, and cellular organdies, comprising:
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selecting a measuring arrangement, comprising (i) a holder, and (ii) a plurality of measurement sites disposed in the holder, each measurement site having a carrier that separates from one another two fluid compartments, the carrier containing an aperture that connects the two compartments, wherein each carrier includes a binding surface adjacent the aperture that is adapted to seal a sample across the aperture, wherein each compartment is in electrical contact with at least one electrode, and wherein the diameter of each aperture immediately adjacent the binding surface is between about 0.3 μ
m and about 7 μ
m;providing a suspension of membranous samples to one of the fluid compartments of at least two measurement sites; sealing a first sample from the suspension across a first aperture in the measuring arrangement; measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the first sample, after the first sample is sealed across the first aperture; sealing a second sample from the suspension across a second aperture in the measuring arrangement; and measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the second sample, after the second sample is sealed across the second aperture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A method for analyzing membranous samples, such as cells, vesicles, and cellular organelles, comprising:
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selecting a measuring arrangement, comprising (i) a carrier having a plurality of apertures and a binding surface adjacent each aperture, each aperture being between about 0.3 μ
m and about 7 μ
m in diameter immediately adjacent the binding surface, (ii) at least two fluid compartments, separated by the carrier, each fluid compartment connected to at least one of the other fluid compartments via at least one of the apertures, and (iii) at least two electrodes, each electrode being in electrical contact with at least one of the fluid compartments;providing a suspension of membranous samples to one or more of the fluid compartments, at least two of the apertures being in fluid contact with a portion of the suspension; sealing a first sample from the suspension across a first aperture selected from the at least two of the apertures, and sealing a second sample from the suspension across a second aperture selected from the at least two of the apertures; and measuring, distinguishably, at least one of an electrical potential and a current across at least a portion of each of the first and second samples, following the step of sealing the samples across the apertures. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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60. A method for analyzing membranous samples, such as cells, vesicles, and cellular organdies, comprising:
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selecting a holder having a plurality of measurement sites, each measurement site having a carrier containing an aperture and a binding surface around the aperture, each aperture being between about 0.3 μ
m and about 7 μ
m in diameter immediately adjacent the binding surface, wherein fluid is disposed on opposite sides of the aperture;providing a suspension of membranous samples to at least two of the measurement sites; sealing a first sample from the suspension across an aperture at a first of the plurality of measurement sites, and a second sample from the suspension across an aperture at a second of the plurahuy of measurement sites; and measuring, distinguishably, at least one of an electrical potential difference and a current across at least a portion of each of the first and second samples, following the step of sealing the samples across the apertures. - View Dependent Claims (61, 62, 63, 64, 65)
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66. A method for analyzing membranous samples, such as cells, vesicles, and cellular organdies, comprising:
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selecting a measuring arrangement, comprising (i) a carrier having a plurality of apertures and a binding surface encircling each aperture, each aperture being between about 0.3 μ
m and about 7 μ
m in diameter immediately adjacent the binding surface, the carrier being adapted so that at least one of the samples can be sealed across at least one of the apertures on the respective binding surface, (ii) at least one sample fluid compartment and at least one reference fluid compartment, in contact via the apertures, and (iii) at least one sample electrode and at least one reference electrode, each sample electrode in contact with at least one of the sample fluid compartments, and each reference electrode in contact with at least one of the reference fluid compartments, wherein the at least one sample electrode and the at least one reference electrode are adapted to apply and/or measure an electrical potential across the apertures;sealing a first sample across a first aperture in the measuring arrangement, and sealing a second sample across a second aperture in the measuring arrangement; and measuring, distinguishably, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of each of the first and second samples, following the step of sealing the samples across the apertures. - View Dependent Claims (67)
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Specification