Genes for plant fatty acid modifying enzymes associated with conjugated double bond formation

• US 7,244,563 B2
• Filed: 11/04/2002
• Issued: 07/17/2007
• Est. Priority Date: 08/20/1998
• Status: Expired due to Fees

• Current Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Ripp, Kevin G., Hitz, William Dean, Carlson, Thomas J., Cahoon, Edgar Benjamin
• Primary Examiner(s): McElwain; Elizabeth F.
• Application Number:
  US10/287,401
• Publication Number:
  US 20030126640A1
• Time in Patent Office: 1,716 Days
• Field of Search: None
• US Class Current: 435/6.14
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 50/30 for swines
• CPC Class Codes: C11B 1/00 Production of fats or fatty oils from raw materials
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/00 Enzymes; Proenzymes; Compositions thereof preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113; Processes for preparing, activating, inhibiting, separating or purifying enzymes preparation of malt C12C1/00
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)

Abstract

The preparation and use of nucleic acid fragments encoding plant fatty acid modifying enzymes associated with conjugated double bond formation or functionally equivalent subfragments thereof are disclosed. Chimeric genes incorporating such nucleic acid fragments or functionally equivalent subfragments thereof or complement thereof and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles.

Claims

1. A method of improving the carcass quality of an animal by supplementing a diet of the animal with animal feed comprising an oil obtained from a seed, wherein the seed is obtained from a plant cell transformed with a chimeric gene comprising an isolated nucleic acid fragment or functionally equivalent subfragment thereof of operably linked to at least one regulatory sequence, further wherein said isolated nucleic acid fragment encodes a plant fatty acid modifying enzyme associated with conjugated double bond formation further wherein said fragment or a functionally equivalent subfragment thereof

(a) hybridizes to the nucleotide sequence set forth in SEQ ID NO:

3 under conditions of moderate stringency;

or

(b) encodes a polypeptide that has at least 45% sequence identity based on the Clustal method of alignment with a polypeptide set forth in SEQ ID NO;

4 or a functionally equivalent subfragment thereof.

2. The method of claim 1 wherein the isolated nucleic acid fragment is isolated from Impatiens balsamina, Momordica charantia, Chrysobalanus icaco, Licania michauxii, and Aleurites fordii.

3. The method of claim 1 or 2 wherein the plant fatty acid modifying enzyme is associated with the formation of at least one fatty acid selected from the group consisting of eleostearic acid and parinaric acid.

4. A method of improving the carcass quality of an animal by supplementing a diet of the animal with animal feed comprising an oil obtained from a seed, wherein the seed is obtained from a plant cell transformed with a chimeric gene comprising an isolated nucleic acid fragment or functionally equivalent subfragment thereof or a complement of the isolated nucleic acid fragment or functionally equivalent subfragment operably linked to at least one regulatory sequence,

further wherein said isolated nucleic acid fragment encodes a plant fatty acid modifying enzyme associated with conjugated double bond formation further wherein said fragment or a functionally equivalent subfragment thereof

(a) hybridizes to the nucleotide sequences set forth in SEQ ID NO:

3 under conditions of moderate stringency, or

(b) encodes a polypeptide that has at least 45% sequence identity with a polypeptide as set forth in SEQ ID NO;

4 or a functionally equivalent subfragment thereof based on the Clustal method of alignment, and wherein the polypeptide is capable of catalyzing the production of at least one fatty acid selected from the group consisting of eleostearic acid and parinaric acid when expressed in a plant cell or yeast transformed with the isolated nucleic acid fragment and said fatty acids are not produced and/or accumulated in the untransformed plant cell or yeast.

5. The method of claim 4, wherein the plant cell comprises a cell from a crop, selected from the group consisting of soybean, Brassica species, corn, peanut, rice, wheat, sunflower, safflower, cotton, and cocoa.