One-dimensional arrays on optical fibers

• US 7,244,572 B1
• Filed: 03/24/2000
• Issued: 07/17/2007
• Est. Priority Date: 03/24/2000
• Status: Expired due to Fees

• Current Assignee: UWM Research Foundation, Inc.
• Original Assignee: WiSys Technology Foundation, Inc.
• Inventors: Geissinger, Peter, Schwabacher, Alan W.
• Primary Examiner(s): SHIBUYA, MARK LANCE
• Application Number:
  US09/535,300
• Time in Patent Office: 2,671 Days
• Field of Search: 435/4, 435/7.1, 435/287.1, 435/288.1, 435/808, 435/288.7, 435/DIG.2, 435/DIG.15, 435/DIG.43, 435/DIG.45, 436/8, 436/578, 436/524, 436/527, 436/164, 436/172, 436/805, 422/57, 422/82.11, 530/300, 530/333, 530/334
• US Class Current: 435/7.1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00515 in the shape of strings
• CPC Class Codes: B01J 2219/00538 in the shape of cylinders
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B

Abstract

Linear arrays of chemosensors or chemical compounds are supported by an optical fiber that allows one to rapidly assay the entire array using changes in optical properties such as fluorescence. The location of the agent along the fiber determines the identity of the agent in these linear arrays. Combinatorial libraries may be constructed on the fiber as well as assayed on the optical fiber. A system and method of analyzing the entire array of agents on an optical fiber using a light source, an optical fiber, and a detector are also described. The time delay between the excitation and detection determines the location being assayed along the fiber and therefore the identity of the agent being assayed. The present invention may find uses in the medical, pharmaceutical, environmental, defense, and food industries.

Claims

1. A chemical testing apparatus comprising:

an optical fiber providing a linear support conducting light along a length between two ends; and

a combinatorial library of probe compounds attached at discrete locations along the length of the optical fiber in a predetermined pattern, the probe compounds positioned to be exposed to target compounds applied to the optical fiber.

2. The chemical testing apparatus of claim 1 wherein the probe compounds are peptides.

3. The chemical testing apparatus of claim 1 further including a light source providing light conducted along the optical fiber to detect modification of the probe compounds during reaction with the target compounds.

4. The chemical testing apparatus of claim 3 wherein the light source is attached to at least one end of the optical fiber to transmit light by internal reflection along the length of the optical fiber to interact with multiple different probe compounds.

5. The chemical testing apparatus of claim 1 wherein the probe compounds are placed on the optical fiber to couple with evanescent waves through the fiber.

6. The chemical testing apparatus of claim 1 wherein the probe compounds repeat with a predetermined spatial pattern.

7. The chemical testing apparatus of claim 1 further including a light sensor receiving light from the optical fiber to distinguish among light interacting with different probe compounds.

8. The chemical testing apparatus of claim 7 further including a means for Fourier analysis of light received from the light sensor.

9. A method of testing an analyte having target compounds, the method comprising the steps of:

(a) preparing an optical fiber with a combinatorial library of probe compounds attached at discrete locations along the length of the optical fiber in a predetermined pattern;

(b) exposing the prepared optical fiber to target compounds in an analyte; and

(c) photometrically analyzing the exposed and prepared optical fibers to detect reaction of the probe compounds with the target compounds from the analyte.

10. The method of claim 9 wherein the probe compounds are peptides.

11. The method of claim 9 further wherein the step of analyzing conducts light along the optical fiber to detect modification of the probe compounds during reaction with the target compounds.

12. The method of claim 11 wherein the light source is attached to at least one end of the optical fiber to transmit light by internal reflection along the length of the optical fiber, whereby light from the light source is capable of interacting with multiple different probe molecules.

13. The method of claim 9 wherein the probe compounds are placed on the optical fiber to couple with evanescent waves through the fiber.

14. The method of claim 9 wherein the probe molecules repeat with a predetermined spatial pattern.

15. The method of claim 9 further including the step of receiving light conducted along the optical fiber at a light sensor capable of distinguishing among light that has interacted with different probe molecules.

16. The method of claim 15 further including the step of conducting a Fourier analysis of the light received by the light sensor.