Polymerase chimeras

• US 7,244,602 B2
• Filed: 06/06/2003
• Issued: 07/17/2007
• Est. Priority Date: 03/13/1998
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Original Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Inventors: Ankenbauer, Waltraud, Sobek, Harald, Schomburg, Dietmar, Frey, Bruno, Villbrandt, Britta
• Primary Examiner(s): HUTSON, RICHARD G
• Application Number:
  US10/456,129
• Publication Number:
  US 20040058362A1
• Time in Patent Office: 1,502 Days
• Field of Search: 435/194, 435/6, 435/91.1, 435/91.2, 536/22.1, 536/23.1
• US Class Current: 435/194
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.

Claims

1. A polymerase chimera comprising the amino acid sequence of SEQ ID NO:

12.

2. The polymerase chimera as claimed in claim 1, wherein the chimera additionally has reverse transcriptase activity.

3. The polymerase of claim 2, wherein histidine tags have been incorporated in the amino acid sequence of the chimera.

4. A kit comprising the polymerase chimera of claim 1.