Aerobic succinate production in bacteria
First Claim
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1. A method of producing succinic acid in an aerobic culture comprising:
- a) supplying a mutant strain of E. coli bacteria, wherein said mutant strain of E. coli bacteria comprises inactivation of sdhAB, ackA-pta, and poxB;
b) contacting the bacteria with a glucose substrate;
c) allowing the bacteria to metabolize the glucose under aerobic conditions; and
d) isolating and recovering the succinic acid from the culture, wherein the bacteria produces succinic acid from the glucose substrate in a ratio of at least 0.6;
1 succinic acid to substrate.
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Abstract
Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
73 Citations
9 Claims
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1. A method of producing succinic acid in an aerobic culture comprising:
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a) supplying a mutant strain of E. coli bacteria, wherein said mutant strain of E. coli bacteria comprises inactivation of sdhAB, ackA-pta, and poxB; b) contacting the bacteria with a glucose substrate; c) allowing the bacteria to metabolize the glucose under aerobic conditions; and d) isolating and recovering the succinic acid from the culture, wherein the bacteria produces succinic acid from the glucose substrate in a ratio of at least 0.6;
1 succinic acid to substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of producing succinic acid in an aerobic culture comprising:
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a) supplying a mutant strain of E. coli bacteria, wherein said mutant strain of E. coli bacteria comprises; i) inactivation of sdhAB, ackA-pta, poxB, ptsG, iclR, and ii) overexpression of pepc; b) contacting the bacteria with a glucose substrate; c) allowing the bacteria to metabolize the glucose under aerobic conditions; and
isolating and recovering the succinic acid from the culture, wherein the bacteria produces succinic acid from the glucose substrate in a ratio of at least 0.9;
1 succinic acid to substrate. - View Dependent Claims (9)
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Specification