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Polychronic laser scanning system and method of use

  • US 7,248,360 B2
  • Filed: 03/25/2005
  • Issued: 07/24/2007
  • Est. Priority Date: 04/02/2004
  • Status: Expired due to Fees
First Claim
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1. A system for performing laser scanning of a liquid sample containing particles, the system comprising:

  • (a) means for scanning an excitation laser beam from an excitation laser across the sample;

    (b) means for focusing the excitation laser beam into the sample;

    (c) means for collecting a light emitted from the sample and its constituents in response to the excitation laser beam;

    (d) means for de-scanning the collected emission light;

    (e) means for spectrally dispersing the collected emission light;

    (f) means for imaging the spectrally dispersed emission light as a spectrum;

    (g) means for detecting the spectrum in one or more detection channels simultaneously according to its wavelength using a two-dimensional detector surface; and

    (h) at least one afocal lens pair in a beam path of the collected emission light between a dichroic mirror and the means for spectrally dispersing.

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