Polychronic laser scanning system and method of use

• US 7,248,360 B2
• Filed: 03/25/2005
• Issued: 07/24/2007
• Est. Priority Date: 04/02/2004
• Status: Expired due to Fees

• Current Assignee: PPD Biomarker Discovery Sciences LLC (CellCarta Biosciences, Inc.)
• Original Assignee: PPD Biomarker Discovery Sciences LLC (CellCarta Biosciences, Inc.)
• Inventors: Kantor, Aaron B., Horchner, Uwe
• Primary Examiner(s): Toatley, Jr.; Gregory J.
• Assistant Examiner(s): Stock, Jr.; Gordon J.
• Application Number:
  US11/090,673
• Publication Number:
  US 20050280817A1
• Time in Patent Office: 851 Days
• Field of Search: None
• US Class Current: 356/318
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/144 Imaging characterised by its optical setup
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons

Abstract

A system for laser scanning provides spectral flexibility needed for the spectroscopic monitoring of highly multiplexed samples, such as cellular and particle assays in whole blood or other suspensions. In accordance with embodiments of the present invention, the system comprises a scanner to direct an excitation laser through a sample, an objective to collect light emitted by the sample in response to the excitation laser, a spectrograph to disperse the emitted light over a plurality of wavelengths as a spectrum, and a charge coupled device for detecting the spectrum. The system can be used with samples having a variety of reporter tags, including one or more SERS tags, fluorescent organic and protein tags, and quantum dot tags. A laser scanning apparatus and method of using the same is also provided.

Claims

1. A system for performing laser scanning of a liquid sample containing particles, the system comprising:

(a) means for scanning an excitation laser beam from an excitation laser across the sample;

(b) means for focusing the excitation laser beam into the sample;

(c) means for collecting a light emitted from the sample and its constituents in response to the excitation laser beam;

(d) means for de-scanning the collected emission light;

(e) means for spectrally dispersing the collected emission light;

(f) means for imaging the spectrally dispersed emission light as a spectrum;

(g) means for detecting the spectrum in one or more detection channels simultaneously according to its wavelength using a two-dimensional detector surface; and

(h) at least one afocal lens pair in a beam path of the collected emission light between a dichroic mirror and the means for spectrally dispersing.

2. The system as claimed in claim 1, wherein the two-dimensional detector surface comprises a charge coupled device.

3. The system as claimed in claim 1, further comprising means for changing a readout pattern of the two-dimensional detector surface in real-time, wherein the means for changing effectively reformats (a) a number of simultaneous detection channels, and (b) a spectral bandpass of at least one of the one or more detection channels.

4. The system as claimed in claim 1, wherein the means for scanning and the means for de-scanning comprise a single galvanometer-mirror.

5. The systeni as claimed in claim 1, wherein the sample comprises suspensions selected from the group consisting of cells, man-made particles, and mixtures thereof.

6. The system as claimed in claim 1, wherein a structure holding the sample is selected from the group consisting of one or more capillaries having a known volume, one or more non-capillary containers comprising well plates, and one or more slides comprising a flat surface.

7. The system as claimed in claim 1, wherein the means for collecting, the means for spectrally dispersing, and the means for imaging provide a magnification appropriate to image the scanned particles onto the two-dimensional detector surface.

8. The system as claimed in claim 1, further comprising a pinhole at an internal focal plane of the at least one afocal lens pair, wherein the pinhole prevents a substantial portion of light emitted from outside a focal plane of the excitation laser beam from reaching the means for spectrally dispersing.

9. The system as claimed in claim 1, wherein the means for spectrally dispersing and the means for imaging is selected from the group consisting of (a) a concave holographic grating, and (b) two toroidal mirrors and a planar diffraction grating.

10. The system as claimed in claim 1, wherein the means for spectrally dispersing is adapted for a spectral range of 200 nm or more beginning from a wavelength of the excitation laser beani and continuing to longer wavelengths.

11. The system as claimed in claim 1, wherein the two-dimensional detector surface comprises a CMOS sensor.

12. The system as claimed in claim 1, wherein, except for the spectrum of the collected emission light emitted by the sample in response to the excitation laser beam, the two-dimensional detector surface is shielded from substantially all light.

13. The system as claimed in claim 1, wherein the two-dimensional detector surface is positioned in a focal plane of the means for imaging such that a dispersion direction is oriented substantially parallel to readout registers and shift registers of the two-dimensional detector surface, wherein the two dimensional detector surface is selected from the group consisting of a charge coupled device and a CMOS sensor.

14. The system as claimed in claim 1, wherein the means for detecting comprises on-chip 2-D binning.

15. The system as claimed in claim 1, wherein a readout pattern of the two-dimensional detector surface can be changed in real-time by reprogramming one or more clock-drivers.

16. The system as claimed in claim 1, wherein the means for scanning comprises means for raster scanning.

17. The system as claimed in claim 16, wherein a galvanometer-mirror implements one axis of the raster-scan across a surface of the sample.

18. The system as claimed in claim 1, wherein the means for focusing and the means for collecting comprise a common objective, wherein the common objective focuses the excitation laser into the sample and confocally collects the light emitted in response to the delivered excitation laser beam.

19. The system as claimed in claim 18, wherein the common objective comprises an F-Theta scan lens.

20. The system as claimed in claim 19, wherein the F-Theta scan lens comprises a focal length of about 6.8 mm, a numerical aperture of about 0.55, and a magnification of about 20.

21. The system as claimed in claim 1, wherein the sample comprises one or more reporter tags, the one or more reporter tags comprising at least one reactive component associated with one or more functional groups of interest of at least one of the particles in the sample, and at least one optically active component for absorbing photons with a wavelength of the excitation laser and emitting photons at a different wavelength.

22. The system as claimed in claim 21, wherein the at least one reactive component of the reporter tag is selected from the group consisting of an antibody, antigen, receptor, hapten, and lectin, and the at least one optically active component of the report tag is selected from the group consisting of an organic dye, a surface enhanced Raman scattering particle, a quantum dot, an up-converting phosphor, a fluorescent protein, a molecular beacon, a luminescent compound or particle and a combination of two or more thereof.

23. The system as claimed in claim 22, wherein the reporter tags are chemically or physically interconnected to a particle of suitable size and the reactive part of the reporter tag is capable of binding soluble factors contained in the sample.

24. The system as claimed in claim 1, wherein the excitation laser comprises a helium-neon gas laser having a wavelength of about 632.8 nm, and wherein the means for detecting is optimal for a wavelength range of between about 620 nm and 850 nm, and wherein the sample comprises whole blood.

25. The system as claimed in claim 24, wherein the sample comprises at least one SERS-particle.

26. An apparatus for performing laser scanning on a sample having particles in a suspension, comprising:

(a) one or more sources of excitation source;

(b) a scanner to direct the excitation source through the sample;

(c) an objective to collect light emitted by the sample in response to the excitation source;

(d) a spectrograph to disperse the emitted light over a plurality of wavelengths as a spectrum;

(e) at least one afocal lens pair between a dichroic beam splitter and the spectrograph, wherein the dichroic beam splitter at least partially divides an excitation path and a detection path; and

(f) a charge coupled device for detecting the spectrum.

27. The apparatus as claimed in claim 26, wherein the charge coupled device produces a readout pattern that can be changed in real-time by reformatting (a) a number of simultaneous detection channels, and (b) a spectral bandpass of at least one of the one or more detection channels.

28. The apparatus as claimed in claim 26, further comprising a pinhole at an internal focal plane of the at least one afocal lens pair, wherein the pinhole prevents a substantial portion of light emifted from outside the focal plane of the excitation source from reaching the spectrograph.

29. The apparatus as claimed in claim 26, wherein the objective, the at least one afocal lens pair, and the spectrograph provide a magnification appropriate to image the scanned particles onto a two-dimensional surface of the charge coupled device.

30. The apparatus as claimed in claim 26, wherein the spectrograph comprises a concave holographic grating.

31. The apparatus as claimed in claim 26, wherein the spectrograph is adapted for a spectral range of 200 nm or more beginning from a wavelength of the excitation source and continuing to longer wavelengths.

32. The apparatus as claimed in claim 26, wherein a readout pattern of the charge coupled device can be changed in real-time by reprogramming one or more clock-drivers of the charge coupled device.

33. The apparatus as claimed in claim 26, wherein the charge coupled device is positioned in a focal plane of the spectrograph such that a dispersion direction is oriented substantially parallel to readout registers and shift registers of the charge coupled device.

34. The apparatus as claimed in claim 26, wherein, except for the spectrum of the collected emission light emitted by the sample in response to the excitation source, the charge coupled device is shielded from substantially all light.

35. The apparatus as claimed in claim 26, wherein the excitation source comprises a helium-neon gas laser having a wavelength of about 632.8 nm, and wherein the charge coupled device is optimal for a wavelength range of between about 620 nm and 850 nm, and wherein the sample comprises whole blood.

36. The apparatus as claimed in claim 26, wherein the scanner comprises a raster scanner.

37. The apparatus as claimed in claim 36, wherein a galvanometer-mirror implements one axis of the raster-scan across a surface of the sample.

38. The apparatus as claimed in claim 26, wherein the objective comprises an F-Theta scan lens to focus the excitation source into the sample and confocally collect the light emitted in response to the delivered excitation source.

39. The system as claimed in claim 38, wherein the F-Theta scan lens comprises a focal length of about 6.8 mm, a numerical aperture of about 0.55, and a magnification of about 20.