Method and apparatus for dynamically monitoring multiple in vivo tissue chromophores
First Claim
1. A method of monitoring methemoglobinemia in a subject comprising the steps of:
- providing a broadband diffuse optical spectroscopy device, the device having a first light source emitting light at a plurality of wavelengths and second light source comprising a broadband light source, first and second source optical fibers optically coupled, respectively, to the first and second light sources, first and second detector optical fibers optically coupled, respectively, to a detector and a spectrometer, the broadband diffuse optical spectroscopy device including a probe that is configured for placement adjacent to the subject'"'"'s skin;
radiating the subject with light from the first light source;
detecting intensity-modulated diffuse reflectance signals using the detector;
determining absorption and reduced scattering coefficients at each wavelength of the first light source;
radiating the subject with radiation from the second light source;
acquiring broadband reflectance measurements using the spectrometer;
determining absolute reflectance spectra based on the broadband reflectance measurements and the absorption and reduced scattering coefficients;
calculating μ
a spectra from the absolute reflectance spectra; and
determining the concentration of methemoglobin, deoxyhemoglobin, and oxyhemoglobin, based on wavelength-dependent extinction coefficient spectra;
calculating a percentage of methemoglobin based on the formula [MetHb]/([Hb-O2]+[Hb-R]+[MetHb]); and
displaying the percentage of methemoglobin on a computer display connected to the broadband diffuse optical spectroscopy device.
2 Assignments
0 Petitions
Accused Products
Abstract
A device and method utilizes a broadband diffuse optical spectroscopy (DOS) system to dynamically calculate the concentrations of multiple chromophores in vivo using a non-invasive probe. The device and method permit dynamic monitoring of multiple in vivo tissue chromophores non-invasively with sensitivities necessary for effective therapeutic monitoring. The device includes a probe containing first and second source optical fibers as well as first and second detector optical fibers. The probe is placed adjacent to a sample of interest and detects reflected light which is passed to a proximally located detector and spectrometer. The concentrations of multiple chromophores are determined in real time. In a preferred embodiment, the multiple tissue chromophores include at least two of methemoglobin (MetHb), deoxyhemoglobin (Hb-R), oxyhemoglobin (Hb-O2), water (H2O), and methylene blue (MB). The device and method can be used quantify and monitor methemoglobin formation in subjects suffering from methemoglobinemia.
19 Citations
17 Claims
-
1. A method of monitoring methemoglobinemia in a subject comprising the steps of:
-
providing a broadband diffuse optical spectroscopy device, the device having a first light source emitting light at a plurality of wavelengths and second light source comprising a broadband light source, first and second source optical fibers optically coupled, respectively, to the first and second light sources, first and second detector optical fibers optically coupled, respectively, to a detector and a spectrometer, the broadband diffuse optical spectroscopy device including a probe that is configured for placement adjacent to the subject'"'"'s skin; radiating the subject with light from the first light source; detecting intensity-modulated diffuse reflectance signals using the detector; determining absorption and reduced scattering coefficients at each wavelength of the first light source; radiating the subject with radiation from the second light source; acquiring broadband reflectance measurements using the spectrometer; determining absolute reflectance spectra based on the broadband reflectance measurements and the absorption and reduced scattering coefficients; calculating μ
a spectra from the absolute reflectance spectra; anddetermining the concentration of methemoglobin, deoxyhemoglobin, and oxyhemoglobin, based on wavelength-dependent extinction coefficient spectra; calculating a percentage of methemoglobin based on the formula [MetHb]/([Hb-O2]+[Hb-R]+[MetHb]); and displaying the percentage of methemoglobin on a computer display connected to the broadband diffuse optical spectroscopy device. - View Dependent Claims (2, 3, 4, 5)
-
-
6. A method of monitoring methemoglobinemia in a subject comprising the steps of:
-
providing a broadband diffuse optical spectroscopy device, the device having a first light source emitting light at a plurality of wavelengths and second light source comprising a broadband light source, a detector, and a spectrometer, the broadband diffuse optical spectroscopy device including a probe that is configured for placement adjacent to the subject'"'"'s skin; radiating the subject with light from the first and second light sources; determining the concentration of methemoglobin, deoxyhemoglobin, and oxyhemoglobin based on wavelength-dependent extinction coefficient spectra obtained from the broadband diffuse optical spectroscopy device; calculating a percentage of methemoglobin based on the formula [MetHb]/([Hb-O2]+[Hb-R]+[MetHb]); and displaying at least one of the concentration and percentage of methemoglobin on a computer display operatively coupled to the broadband diffuse optical spectroscopy device. - View Dependent Claims (7, 8, 9, 10, 11)
-
-
12. A method of treating methemoglobinemia in a subject comprising the steps of:
-
providing a broadband diffuse optical spectroscopy device, the device having a first light source emitting light at a plurality of wavelengths and second light source comprising a broadband light source, a detector, and a spectrometer, the broadband diffuses optical spectroscopy device including a probe that is configured for placement adjacent to the subject'"'"'s skin; administering methylene blue to the subject; radiating the subject with light from the first and second light sources; determining the concentration of methemoglobin, deoxyhemoglobin, oxyhemoglobin and methylene blue based on wavelength-dependent extinction coefficient spectra obtained from the broadband diffuse optical spectroscopy device; calculating a percentage of methemoglobin based on the formula [MetHb]/([Hb-O2]+[Hb-R]+[MetHb]); and adjusting the administration of methylene based at least in part on the calculated percentage of methemoglobin. - View Dependent Claims (13, 14, 15, 16, 17)
-
Specification