Genes with ES cell-specific expression

• US 7,250,255 B2
• Filed: 05/31/2002
• Issued: 07/31/2007
• Est. Priority Date: 05/31/2001
• Status: Expired due to Term

• Current Assignee: Shinya Yamanaka, Sumitomo Pharma Co Ltd (Sumitomo Chemical Company Limited)
• Original Assignee: Shinya Yamanaka, Dainippon Sumitomo Pharma Company Limited (Sumitomo Chemical Company Limited)
• Inventors: Yamanaka, Shinya
• Primary Examiner(s): Forman; B J
• Assistant Examiner(s): KAPUSHOC, STEPHEN THOMAS
• Application Number:
  US10/479,334
• Publication Number:
  US 20040137460A1
• Time in Patent Office: 1,887 Days
• Field of Search: None
• US Class Current: 435/6.14
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12Q 1/6881 for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The present invention relates to a probe for selecting ES cells, which characteristically contains one of DNAs having base sequences depicted in SEQ ID Nos; 1, 2, 3, 4, 5, 6, 7 and 8, or DNAs having base sequences depicted in SEQ ID Nos; 9, 11, 13, 15, 17, 19, 21, 23 and 41 and a screening method of ES cell using this probe. Preparation of a probe for selecting ES cells becomes feasible by identifying plural gene with ES cell-specific expressions (ECAT genes) and using the information of the base sequences of these gene groups. Efficient selection of ES cell enables supply of a large amount of ES cell expected to be applicable to regenerative medicine.

Claims

1. An isolated nucleic acid comprising a DNA comprising SEQ ID NO:

15.

2. A vector comprising the nucleic acid of claim 1.

3. An isolated cell comprising the vector of claim 2.

4. An isolated probe for selecting mouse ES cells, wherein the probe comprises the nucleic acid of claim 1.