Methods for prevention of surface adsorption of biological materials to capillary walls in microchannels
First Claim
1. A method of reducing adsorption of one or more materials to an interior surface of a microchannel, the method comprising flowing the one or more macromolecules in a fluid in the microchannel, and concomitantly flowing a colloidal material through the fluid in the microchannel at a sufficient concentration to bind to the one or more materials and thereby prevent the materials from binding to the interior surface of the microchannel.
2 Assignments
0 Petitions
Accused Products
Abstract
Methods for reducing surface adsorption of biological materials to the walls of microfluidic conduits in microscale devices are provided. In an example of the methods, one or more colloidal-size particles, such as colloidal silica particles, are flowed in a fluid within the microfluidic conduit in the presence of one or more adherent biological materials (such as one or more proteins, cells, carbohydrates, nucleic acids, lipids and the like) to adsorb to the materials and prevent them from binding to the capillary walls of the microfluidic conduit. Other adsorption inhibition agents such as detergents and nonaqueous solvents can be used alone or in combination with colloidal particles to reduce surface adsorption in microfluidic conduits.
-
Citations
28 Claims
- 1. A method of reducing adsorption of one or more materials to an interior surface of a microchannel, the method comprising flowing the one or more macromolecules in a fluid in the microchannel, and concomitantly flowing a colloidal material through the fluid in the microchannel at a sufficient concentration to bind to the one or more materials and thereby prevent the materials from binding to the interior surface of the microchannel.
Specification