In Vitro sorting method
First Claim
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1. A method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of:
- (a) expressing the genetic elements to produce their respective gene products such that the gene products are linked to the genes encoding them;
(b) compartmentalizing the genetic elements into microcapsules;
(c) sorting the genetic elements which produce gene product(s) having the desired activity.
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Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: compartmentalizing genetic elements into microcapsules; expressing the genetic elements to produce their respective gene products within the microcapsules; sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.
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Citations
21 Claims
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1. A method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of:
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(a) expressing the genetic elements to produce their respective gene products such that the gene products are linked to the genes encoding them; (b) compartmentalizing the genetic elements into microcapsules; (c) sorting the genetic elements which produce gene product(s) having the desired activity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for preparing a gene product, comprising the steps of:
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(a) preparing a genetic clement encoding the gene product; (b) expressing the genetic elements to produce their respective gene products such that the gene products are linked to the genetic elements encoding them; (c) compartmentalizing genetic elements into microcapsules; (d) sorting the genetic elements which produce the gene product(s) having the desired activity; and (e) expressing the gene product having the desired activity.
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Specification