Chromatin regulator genes

• US 7,252,968 B2
• Filed: 02/09/2004
• Issued: 08/07/2007
• Est. Priority Date: 05/10/1995
• Status: Expired due to Fees

• Current Assignee: Boehringer Ingelheim International GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Boehringer Ingelheim International GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Inventors: Rea, Stephen, Jenuwein, Thomas, Laible, Go, O'Carroll, Donal, Eisenhaber, Frank
• Primary Examiner(s): Martinell; James
• Application Number:
  US10/773,302
• Publication Number:
  US 20050089880A1
• Time in Patent Office: 1,275 Days
• Field of Search: None
• US Class Current: 435/69.1
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/4702 Regulators; Modulating activity
• CPC Class Codes: Y02A 90/10 Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Abstract

The invention concerns the deregulation of the chromatin-regulator genes which have a SET domain, such deregulation being of importance in certain cancer conditions. These genes can be used in the diagnosis and therapy of such conditions.

Claims

1. An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:

(a) a polynucleotide encoding amino acids 1 to 746 of SEQ ID NO;

2;

(b) a polynucleotide enoding a protein containing amino acids of SEQ ID NO;

2, wherein a stop codon shortens the protein by 47 C-terminal amino acids; and

with

a polynucleotide encoding a protein containing amino acids 1–

699 of SEQ ID NO;

2; and

(c) a polynucleotide complementary to the polynucleotide of (a) or (b).

2. The nucleic acid molecule of claim 1, wherein said polynucleotide is (a).

3. The nucleic acid molecule of claim 2, wherein said polynucleotide comprises nucleotides 90–

2330 of SEQ ID NO;

1.

4. The nucleic acid molecule of claim 1, wherein said polynucleotide is (b).

5. The nucleic acid molecule of claim 1, wherein said polynucleotide is (c).

6. A vector comprising the isolated nucleic acid molecule of claim 1.

7. A method of producing a vector comprising inserting the isolated nucleic acid molecule of claim 1 into a vector.

8. An isolated recombinant DNA molecule comprising:

(a) the isolated DNA molecule of claim 1; and

(b) expression control sequences.

9. A host cell comprising the isolated nucleic acid molecule of claim 1.

10. The host cell of claim 9 that is prokaryotic.

11. The host cell of claim 9 that is eukaryotic.

12. The host cell of claim 9 wherein said isolated nucleic acid molecule is operably associated with a heterologous regulatory sequence.

13. A method of producing a polypeptide comprising culturing the host cell of claim 9 under conditions such that said polypeptide is expressed, and recovering said polypeptide.