Device and method for manufacturing bead array, and method for detecting target substance

• US 7,253,006 B2
• Filed: 08/31/2004
• Issued: 08/07/2007
• Est. Priority Date: 09/01/2003
• Status: Expired due to Fees

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: Takagi, Fumio
• Primary Examiner(s): Gordon; Brian R.
• Application Number:
  US10/931,258
• Publication Number:
  US 20050079592A1
• Time in Patent Office: 1,071 Days
• Field of Search: 422 99-100, 422/69, 422/68.1, 436/174, 436/180, 436/63, 435/174
• US Class Current: 436/180
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00466 in a slurry
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00545 Colours
• CPC Class Codes: B01J 2219/00554 Physical means
• CPC Class Codes: B01J 2219/00563 Magnetic means
• CPC Class Codes: B01J 2219/00574 radioactive
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A device for manufacturing a bead array is provided including a container, a liquid including a bead having a biological substance fixed on a surface of the bead, and means for ejecting the liquid including the bead on a desired position on a solid-phase base. A method for manufacturing a bead array is also provided including: containing the liquid including the bead in a container; selectively ejecting the liquid on a desired position on the solid-phase base; and forming a reactive region where the liquid is ejected.

Claims

1. A method for manufacturing a bead array, comprising:

storing a liquid including a bead in a container, the bead having a biological substance fixed on a surface thereof;

ejecting the liquid including the bead to a desired position on a solid-phase base by ink jetting the liquid including the bead;

forming a reactive region on the solid-phase base having a cover plate joined to the base surface and a resin film disposed along the periphery of the base surface to form the reactive region within a reactive chamber in which wells join one another through gaps smaller than the diameter of the beads, where the liquid including the bead is ejected;

separately storing another liquid including another bead in the container, the another bead of the another liquid having the biological substance fixed on a surface thereof and including an identifier, wherein the identifier is at least one of bead size and bead density; and

ejecting the another liquid on the solid-phase base by ink jetting the another liquid.

2. The method for manufacturing a bead array according to claim 1, wherein the reactive region includes a plurality of beads, each of the beads having a single type of biological substance fixed thereto.

3. The method for manufacturing a bead array according to claim 1, wherein the reactive region includes a plurality of bead groups, each bead group having a single type of biological substance fixed to each bead of the group.

4. The method for manufacturing a bead array according to claim 1, wherein the biological substance comprises any one of nucleic acid and protein.

5. The method for manufacturing a bead array according to claim 1, wherein a diameter of the bead is from about 0.02 to about 10 μ

m inclusive.

6. The method for manufacturing a bead array according to claim 1, wherein a diameter of the bead is from about 0.02 to about 5 μ

m inclusive.

7. The method for manufacturing a bead array according to claim 1, wherein the solid-phase base comprises a microtiter plate.

8. The method for manufacturing a bead array according to claim 1, wherein the solid-phase base and the cover plate comprise glass.

9. A method for manufacturing a bead array, comprising:

storing a liquid including a bead in a container, the bead having a biological substance fixed on a surface thereof;

ejecting the liquid including the bead to a desired position on a solid-phase base by ink jetting the liquid including the bead;

forming a reactive region on the solid-phase base where the liquid including the bead is ejected by joining a cover plate to the base surface covered with a resin film disposed along the periphery of the base surface to form the reactive region within a reactive chamber in which wells join one another through gaps smaller than the diameter of the beads;

separately storing another liquid including another bead in the container, the another bead of the another liquid having the biological substance fixed on a surface thereof and including an identifier, wherein the identifier comprises at least one of bead size and bead density used in combination with at least one of a bead coloring, a fluorescent marker, a magnetic marker, and a radiation marker; and

ejecting the another liquid on the solid-phase base by ink jetting the another liquid.