Reactive probes for two-photon fluorescent imaging and sensing
1 Assignment
0 Petitions
Accused Products
Abstract
Fluorescent dyes and probes are key components in multiphoton based fluorescence microscopy imaging of biological samples. In order to address the demand for better performing dyes for two-photon based imaging, a new series of reactive fluorophores tailored for multiphoton imaging has been disclosed. These fluorophores are based upon the fluorene ring system, known to exhibit high fluorescence quantum yields, typically >0.7, and possess high photostability. They have been functionalized with moieties to act, e.g., as efficient amine-reactive fluorescent probes for the covalent attachment onto, e.g., proteins and antibodies. The synthesis and the single-photon spectral characteristics, as well as measured two-photon absorption cross sections of the reactive fluorophores in solution are presented. Spectral characterizations of bovine serum albumin (BSA) conjugated with the new reactive probe is presented.
-
Citations
9 Claims
-
1. A reactive fluorophore of the structural formula
- 2. A dye adduct of the structural formula
-
3. A BSA model bioconjugate of the structural formula
-
4. A hydrophilic amine-reactive fluorophore of the structural formula
-
6. A hydrophilic protein bioconjugate of the structural formula
-
7. A Generic Two-Photon Reactive Probe Structure of the formula
-
8. A two-photon fluorphore labeled protein of the formula
-
9. A two-photon fluorphore labeled antibody of the formula
Specification
-
- Resources
-
Current AssigneeUniversity of Central Florida (State University System of Florida)
-
Original AssigneeUniversity of Central Florida (State University System of Florida)
-
InventorsBelfield, Kevin D., Schafer, Katherine J.
-
Primary Examiner(s)McKane; Joseph K.
-
Assistant Examiner(s)Barker; Michael P
-
Application NumberUS11/128,497Time in Patent Office816 DaysField of Search548/160US Class Current548/160CPC Class CodesC07D 277/66 with aromatic rings or ring...
