Analyte assay using particulate labels

• US 7,255,995 B2
• Filed: 09/22/2004
• Issued: 08/14/2007
• Est. Priority Date: 04/25/1996
• Status: Expired due to Fees

• Current Assignee: Invitrogen Corp. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Invitrogen Corp. (Thermo Fisher Scientific Incorporated)
• Inventors: Kohne, David E., Yguerabide, Juan, Jackson, Jeffrey T., Yguerabide, Evangelina E.
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US10/947,636
• Publication Number:
  US 20050112784A1
• Time in Patent Office: 1,056 Days
• Field of Search: 435/6, 536/23.1, 536/24.3
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2563/103 luminescence
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 15/1425 using an analyser being characterised by its control arrangement
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/0038 Investigating nanoparticles
• CPC Class Codes: G01N 2015/1493 Particle size
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/585 with a particulate label, e.g. coloured latex
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/817 Steroids or hormones

Abstract

Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.

Claims

1. A method for comparing and identifying nucleic acid sequences in two or more collections of labeled nucleic acids, the method comprising:

providing a plurality of nucleic acid probes bound to a solid surface, at least some of said plurality comprising a nucleic acid complementary to a nucleic acid sequence in said collections to be compared;

contacting the nucleic acid probes with a first collection of labeled nucleic acids having a first type of label, and at least a second collection of labeled nucleic acids having a second type of label;

wherein said first and second types of labels are light scattering particles and are distinguishable from each other and wherein said labeled nucleic acids in said first and second collections bind to said probes; and

detecting the binding for each of the first and at least second labeled nucleic acids to the nucleic acid probes;

thereby comparing and identifying said nucleic acid sequences in said two or more collections.

2. The method as recited in claim 1 wherein the labeled nucleic acids are from two cell types and are separately labeled with first and second light scattering particle types.

3. The method as recited in claim 2 wherein said first and second light scattering particle types have different colors of scattered light.

4. The method as recited in claim 3 wherein said colors are red and green.

5. The method as recited in claim 1 wherein said nucleic acid probes are provided by synthesis on the solid surface.

6. The method of claim 1 wherein the nucleic acids are DNA.

7. The method of claim 1 wherein the nucleic acids are cDNA.

8. The method of claim 1 wherein the first and second labeled nucleic acids comprise human DNA.

9. The method of claim 1 wherein the nucleic acids are greater than about 25 nucleotides in complexity.

10. The method of claim 1 wherein the solid surface is glass.

11. The method of claim 1 wherein the first labeled nucleic acids comprise mRNA or cDNA from a test cell and the second labeled nucleic acids comprise mRNA or cDNA from a reference cell.

12. The method of claim 1 wherein the first labeled nucleic acids are from a test genome and the second labeled nucleic acids are from a normal reference genome.

13. The method of claim 1 wherein the first labeled nucleic acids are from a tumor.

14. The method according to claim 1 wherein the plurality of probes includes more than 100 probes per square centimeter bound to said solid surface.

15. The method according to claim 1 wherein the plurality of probes includes more than 1000 probes per square centimeter bound to said solid surface.

16. The method according to claim 1 wherein the plurality of probes includes more than 10,000 probes per square centimeter bound to said solid surface.