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Recycling system for manipulation of intracellular NADH availability

  • US 7,256,016 B2
  • Filed: 11/01/2002
  • Issued: 08/14/2007
  • Est. Priority Date: 11/02/2001
  • Status: Active Grant
First Claim
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1. An isolated engineered cell, comprising a recombinant NADH-recycling system having:

  • (a) a native cofactor-independent formate dehydrogenase genetically engineered to reduce its enzymatic activity as compared with the activity in a non-engineered cell, and(b) an expression construct comprising an expressible gene encoding a heterologous NAD+-dependent formate dehydrogenase (FDH) that increases the enzymatic activity of NAD+-dependent FDH in the cell as compared with the activity of NAD+-dependent FDH in a non-engineered cell,wherein said FDH converts NAD+ to NADH in the presence of formate.

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