Method and device for identifying a marker using a detector substance

• US 7,256,051 B2
• Filed: 07/22/2002
• Issued: 08/14/2007
• Est. Priority Date: 08/03/2001
• Status: Expired due to Fees

• Current Assignee: November Aktiengesellschaft Gesellschaft Fur Molekulare Medizin
• Original Assignee: Siemens AG
• Inventors: Kaiser, Joachim
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): JARRETT, LORE RAMILLANO
• Application Number:
  US10/398,227
• Publication Number:
  US 20040035938A1
• Time in Patent Office: 1,849 Days
• Field of Search: 436/172, 436/20, 436/518, 235/462
• US Class Current: 436/172
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G06K 7/12 using a selected wavelength, e.g. to sense red marks and ignore blue marks

Abstract

In order to identify a marking which is arranged on a body and contains a marking substance with a specific property, the marking initially is scanned by use of radiation. The marking substance is then brought into contact with a detector substance having a specific property corresponding to the marking substance. A combination of the marking and detector substances which results because of the corresponding specific properties, exhibits fluorescent behavior. The marking is then scanned by use of the radiation in a second measurement, and identified by based upon the results of the two measurements.

Claims

1. A method for identifying a marking which is arranged on a body and contains a marking substance with a specific property, comprising:

scanning the marking by use of radiation in a first measurement and determining a reference therefrom;

bringing the marking substance into contact with a detector substance having a specific property corresponding to the marking substance, so that a combination of the marking and detector substances exhibits fluorescent behavior;

scanning the marking by use of the radiation in a second measurement; and

identifying the marking by using the reference and a result of the second measurement, and wherein

scanning the marking by use of radiation in a first measurement, bringing the marking substance into contact with a detector substance, and scanning the marking by use of the radiation in a second measurement is carried out in chronological succession.

2. The method as claimed in claim 1, wherein bringing a device in contact with the marking includes moving the device across the marking for the first and second measurement.

3. The method as claimed in claim 1, wherein bringing a device in contact with the marking includes moving the device across the marking for the first and second measurement.

4. The method as claimed in claim 3, wherein the device is moved manually.

5. The method as claimed in claim 3, wherein the device is moved across the marking, first in a first direction and then in a second direction opposite the first direction.

6. The method as claimed in claim 5, wherein the first measurement is carried out during the movement in the first direction.

7. The method as claimed in claim 6, wherein the second measurement is carried out during the movement in the second direction.

8. The method as claimed in claim 5, wherein the contact is brought about during the movement in the first direction.

9. The method as claimed in claim 8, wherein the second measurement is carried out during the movement in the second direction.

10. The method as claimed in claim 5, wherein the second measurement is carried out during the movement in the second direction.

11. The method as claimed in claim 3, wherein the device is brought into direct contact with the marking during the movement.

12. The method as claimed in claim 3, wherein a position of the device is recorded during the movement.

13. The method as claimed in claim 3, wherein a first and a second nucleotide sequence are respectively used as the detector substance and the marking substance.

14. The method as claimed in one claim 3, wherein a detector substance having a fluorophore component is used.

15. The method as claimed in claim 1, wherein the fluorescent behavior of the marking is evaluated with the aid of the first measurement before the marking substance is brought into contact with the detector substance.