Method for detection of pathogenic microorganisms
First Claim
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1. A method comprising:
- a) illuminating a sample with substantially monochromatic light,b) illuminating the sample with broad band light;
c) collecting a spectrum of Raman scattered light from the sample;
d) collecting a spectrum of light transmitted through the sample in a near infrared spectral range;
e) forming a first spatially accurate wavelength resolved image of the sample using Raman scattered light and a second spatially accurate wavelength resolved image using light transmitted through the sample;
f) analyzing the first spatially accurate wavelength resolved image and the second spatially accurate wavelength resolved image for patterns characteristic of a pathogenic microorganism; and
(g) based on said analyzing, identifying the pathogenic microorganism.
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Abstract
A method for the detection and identification of pathogenic microorganisms using Raman scattered light and transmitted light in the near infrared spectral region. The method may include passing the Raman scattered light and transmitted light through a FAST fiber array spectral translator.
44 Citations
24 Claims
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1. A method comprising:
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a) illuminating a sample with substantially monochromatic light, b) illuminating the sample with broad band light; c) collecting a spectrum of Raman scattered light from the sample; d) collecting a spectrum of light transmitted through the sample in a near infrared spectral range; e) forming a first spatially accurate wavelength resolved image of the sample using Raman scattered light and a second spatially accurate wavelength resolved image using light transmitted through the sample; f) analyzing the first spatially accurate wavelength resolved image and the second spatially accurate wavelength resolved image for patterns characteristic of a pathogenic microorganism; and (g) based on said analyzing, identifying the pathogenic microorganism. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method comprising:
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a) illuminating a sample with substantially monochromatic light, b) illuminating the sample with broad band light; c) passing the Raman scattered light and light transmitted through the sample through a FAST fiber array spectral translator; d) collecting a spectrum of Raman scattered light from the sample; e) collecting a spectrum of light transmitted through the sample in a near infrared spectral range; f) forming a first spatially accurate wavelength resolved image of the sample using Raman scattered light and a second spatially accurate wavelength resolved image using light transmitted through the sample; g) analyzing the first spatially accurate wavelength resolved image and the second spatially accurate wavelength resolved image for patterns characteristic of a pathogenic microorganism; and (h) based on said analyzing, identifying the pathogenic microorganism. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification