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Serum biomarkers of Hepatitis B Virus infected liver and methods for detection thereof

  • US 7,257,365 B2
  • Filed: 04/26/2004
  • Issued: 08/14/2007
  • Est. Priority Date: 04/25/2003
  • Status: Active Grant
First Claim
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1. A method for detecting the presence of Hepatitis B Virus (HBV) infection in a patient being tested for HBV infection, comprising:

  • obtaining a serum sample from the patient;

    subjecting the sample to 2D protein gel electrophoresis to separate proteins contained therein on a 2D electrophoresis gel;

    staining proteins separated on the electrophoresis gel;

    scanning the image of stained proteins into an image analysis scanner to obtain an image of serum protein expression profile; and

    comparing the serum protein expression profile consisting of apolipoprotein A-I (apoA-I), apolipoprotein A-I fragments, haptoglobin β

    chain, haptoglobin cleaved β

    -chain, haptoglobin 2 chain, apolipoprotein A-IV (apoA-IV), transthyretin, α

    1-anitrypsin, α

    1-antitrypsin fragments/isoforms, and DNA topoisomerases II (topo-II) obtained from said patient to the protein expression profile obtained from HBV-negative and HBV-infected patients, wherein an altered said protein expression profile compared to HBV-negative patient profiles indicates the presence of HBV infection in the patient.

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