Electrochemical biosensors

• US 7,258,769 B2
• Filed: 04/17/2002
• Issued: 08/21/2007
• Est. Priority Date: 12/24/2001
• Status: Active Grant

• Current Assignee: i-SENS, Inc.
• Original Assignee: i-SENS, Inc.
• Inventors: Kim, Ju-Yong, Cui, Gang, Kim, Moon-Hwan
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US10/416,237
• Publication Number:
  US 20040045821A1
• Time in Patent Office: 1,952 Days
• Field of Search: 20440301-40315
• US Class Current: 204/403.14
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

There is provided electrochemical biosensors with a sample introducing part, comprising a sample introducing passage, an air discharge passage, and a void. The sample introducing passage communicates with the air discharge passage, and the void is formed at the point of communication. Also, disclosed is the electrochemical biosensor with the said sample introducing part and a fluidity determining electrode.

Claims

1. An electrochemical biosensor with a sample introducing part, the sample introducing part comprising a sample introducing passage, an air discharge passage, and a void, wherein the sample introducing passage communicates with the air discharge passage in a substantially perpendicular manner, wherein the void is formed at the point of communication from the sample introducing passage and wherein the ratio of the width of the air discharge passage to that of the sample introducing passage is no more than 1:

2.

2. The electrochemical biosensor according to claim 1, wherein the ratio of the width of the air discharge passage to that of the sample introducing passage is in the range of 1:

5 to 1;

2.

3. The electrochemical biosensor according to claim 1, wherein the sample introducing part has a capacity to introduce 0.1-3.0 μ

l of a sample.

4. The electrochemical biosensor according to claim 1, wherein the sample introducing part has a capacity to introduce 0.11.0 μ

l of a sample.

5. The electrochemical biosensor according to claim 1, wherein the sample introducing part has a capacity to introduce 0.3-0.7 μ

l of a sample.

6. The electrochemical biosensor according to claim 1, wherein the sample introducing passage communicates with the air discharge passage at an angle of 90°

.

7. The electrochemical biosensor according to claim 1, further comprising an oxidase selected from the group consisting of glucose oxidase, lactate oxidase, cholesterol oxidase, glutamate oxidase, horseradish peroxidase, and alcohol oxidase.

8. The electrochemical biosensor according to claim 1, further comprising an electron transfer mediator selected from the group consisting of hexaamineruthenium (III) chloride, potassium ferricyanide, potassium ferrocyanide, dimethylferrocene, ferricinium, ferocene-monocarboxylic acid, 7,7,8,8-tetracyanoquinodimethane, tetrathiafulvalene, nickelocene, N-methylacidinium, tetrathiatetracene, N-methylphenazinium, hydroquinone, 3-dimethylaminobenzoic acid, 3-methyl-2-benzothiozolinone hydrazone, 2-methoxy-4-allylphenol, 4-aminoantipyrin, dimethylaniline, 4-aminoantipyrene, 4-methoxynaphthol, 3,3′

,5,5′

-tetramethylbenzidine, 2,2-azino-di-[3-ethylbenzthiazoline sulfonate], o-dianisidine, o-toluidine, 2,4-dichloro phenol, 4-aminophenazone, benzidine and Prussian blue.

9. The electrochemical biosensor according to claim 8, wherein the electron transfer mediator is hexaamineruthenium (III) chloride.

10. The electrochemical biosensor according to claim 1, wherein the biosensor is a flat type biosensor and further comprises:

a sample introducing spacer;

a base substrate, coupled to said sample introducing spacer, on a surface of which a working electrode and a reference electrode are printed and an oxidase and an electron transfer mediator are provided; and

a cover plate, pressed to said sample introducing spacer, for forming the sample introducing channel,

wherein the sample introducing part is formed in one end of said sample introducing spacer.

11. The electrochemical biosensor according to claim 1, wherein the biosensor is a converse type biosensor and further comprises:

a sample introducing spacer;

a base plate, coupled with said sample introducing spacer, on a surface of which a working electrode and an electrode connector are printed and an oxidase and an electron transfer mediator are provided; and

a cover plate, pressed to said sample introducing spacer, on inner surface of which a reference electrode and an electrode connector are printed,

wherein the sample introducing part is formed in one end of said sample introducing spacer.

12. The electrochemical biosensor according to claim 1, wherein the biosensor is a differential flat type biosensor and further comprises:

a pair of sample introducing spacers;

a base plate, coupled between said sample introducing spacers, on both surfaces of which a pair of working and reference electrodes are printed, respectively, and an oxidase and an electron transfer mediator on one side, and BSA and an electron transfer mediator on the other side are provided, respectively; and

a pair of cover plates are pressed to both surfaces of said sample introducing spacers for forming the sample introducing channels,

wherein the sample introducing parts are formed in one end of each of said sample introducing spacers.

13. The electrochemical biosensor according to claim 1, wherein the biosensor is a differential converse type biosensor and further comprises:

a pair of sample introducing spacers;

a base plate, coupled between said sample introducing spacers, on both surfaces of which a working electrode is printed, respectively, and an oxidase and an electron transfer mediator on one side, and BSA and an electron transfer mediator on the other side are provided, respectively; and

a pair of cover plates having reference electrode and electrode connection part on inner surface are pressed to both said sample introducing spacers to form the sample introducing channels,

wherein the sample introducing parts are formed in one end of each of said sample introducing spacers.

14. The electrochemical biosensor according to claim 1, wherein the biosensor is a converse type biosensor and further comprises:

a sample introducing spacer;

a base plate, coupled with said sample introducing spacer, on a surface of which a working electrode and an electrode connector, and a fluidity determining electrode are printed, and an oxidase and an electron transfer mediator are provided; and

a cover plate, pressed to said sample introducing spacer, on inner surface of which a reference electrode and an electrode connector are printed,

wherein the sample introducing part is formed in one end of said sample introducing spacer.