Polynucleotide encoding a novel acyl coenzyme A, monoacylglycerol acyltransferase-3 (MGAT3), and uses thereof

• US 7,259,002 B2
• Filed: 01/21/2004
• Issued: 08/21/2007
• Est. Priority Date: 01/21/2003
• Status: Active Grant

• Current Assignee: Bristol-Myers Squibb Company
• Original Assignee: Bristol-Myers Squibb Company
• Inventors: Chen, Jian, Feder, John N., Nelson, Thomas C., Meegalla, Rupalie, Cheng, Dong, Ramaker, Michael
• Primary Examiner(s): Saidha; Tekchand
• Application Number:
  US10/761,905
• Publication Number:
  US 20040223959A1
• Time in Patent Office: 1,308 Days
• Field of Search: 435/193, 435/252.3, 435/320.1, 536/23.2
• US Class Current: 435/193
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)

Abstract

The present invention provides novel polynucleotides encoding MGAT3 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing MGAT3 polypeptides, fragments, and homologues thereof. The invention further relates to diagnostic and therapeutic methods for applying these novel MGAT3 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides, such as obesity. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.

Claims

1. An isolated nucleic acid molecule comprising the polynucleotide of SEQ ID NO:

1.

2. A recombinant vector comprising said isolated nucleic acid molecule of claim 1.

3. An isolated recombinant host cell comprising said recombinant vector of claim 2.

4. An isolated recombinant host cell comprising a polypeptide encoded by the isolated nucleic acid of claim 1.

5. A method of making an isolated polypeptide comprising:

(a) culturing said recombinant host cell of claim 3 under conditions such that said polypeptide is expressed; and

(b) recovering said polypeptide.

6. The nucleic acid molecule of claim 1 wherein the nucleic acid is included in at least one of ATCC Deposit numbers PTA-4454 or PTA-4803.