Multiple sampling device and method for investigating biological systems

• US 7,259,019 B2
• Filed: 08/19/2005
• Issued: 08/21/2007
• Est. Priority Date: 03/11/2002
• Status: Expired due to Term

• Current Assignee: Heather L. Lord, Janusz B. Pawliszyn, Marcel Musteata
• Original Assignee: Heather L. Lord, Janusz B. Pawliszyn, Marcel Musteata
• Inventors: Pawliszyn, Janusz B., Musteata, Marcel, Lord, Heather L.
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): DiRamio; Jacqueline A.
• Application Number:
  US11/206,804
• Publication Number:
  US 20050276727A1
• Time in Patent Office: 732 Days
• Field of Search: 436/177, 436/178, 435/287.1, 435/287.2, 435/287.9, 422/57, 422/58, 422/102, 422/104, 422/119, 422/101, 427/8, 427/180, 427/181
• US Class Current: 436/178
• CPC Class Codes: A61B 10/0045 Devices for taking samples of body liquids devices for taking blood samples A61B5/15
• CPC Class Codes: A61B 17/3403 Needle locating or guiding means guides for suture needles A61B17/0482; guiding or tracking by nuclear magnetic resonance G01R33/285
• CPC Class Codes: A61B 5/14514 using means for aiding extraction of interstitial fluid, e.g. microneedles or suction
• CPC Class Codes: A61B 5/150022 for capillary blood or interstitial fluid
• CPC Class Codes: A61B 5/15003 for venous or arterial blood
• CPC Class Codes: A61B 5/150099 by negative pressure, other than vacuum extraction into a syringe by pulling on the piston rod or into pre-evacuated tubes
• CPC Class Codes: A61B 5/150229 Pumps for assisting the blood sampling
• CPC Class Codes: A61B 5/150389 Hollow piercing elements, e.g. canulas, needles, for piercing the skin
• CPC Class Codes: A61B 5/150503 Single-ended needles hollow piercing element tip design A61B5/150396, needle sets for catheters A61M25/06, needles for bringing media into the body A61M5/32
• CPC Class Codes: A61B 5/151 Devices specially adapted for taking samples of capillary blood, e.g. by lancets , needles or blades
• CPC Class Codes: A61B 5/153 Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
• CPC Class Codes: A61B 5/417 the bone marrow
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 2001/4016 being a selective membrane, e.g. dialysis or osmosis
• CPC Class Codes: G01N 2030/009 Extraction
• CPC Class Codes: G01N 2030/062 : extracting sample from raw material
• CPC Class Codes: G01N 2035/1053 : for separating part of the liquid, e.g. filters, extraction phase
• CPC Class Codes: G01N 35/028 : having reaction cells in the form of microtitration plates
• CPC Class Codes: H01J 49/0431 : for liquid samples interfaces to liquid chromatographs G01N30/7233
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 : including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Multiwell plate applications are describe for use with a plurality of fibres having an extraction phase coated thereon in combination with a positioning device. The device and method described are able to perform adsorption of components of interest from a biological system in high volume.

Claims

1. A solid-phase microextraction device for measuring or identifying one or more components of interest from liquid samples arranged in a plurality of wells in a multiwell plate, said device comprising:

a plurality of non-porous fibres, each having a distal end at least partially coated with a polymeric extraction phase for absorbing or adsorbing the component of interest from the liquid samples; and

a positioning device for guiding the coated distal end of said non-porous fibres into a submerged position within the plurality of wells of a multiwell plate, and for removing said non-porous fibres from said wells;

said positioning device comprising;

(i) a mounting plate for mounting each of a plurality of non-porous fibres in a position corresponding to a counterpart well on said multiwell plate, the non-porous fibres being retractably mounted within the mounting plate; and

(ii) a guide for moving said mounting plate so the coated distal end of each of said non-porous fibres is submerged in a well of the multiwell plate, for retracting said non-porous fibres from said wells, and for positioning said non-porous fibres into a multiwell plate containing desorption solvent or into an analytical instrument.

2. The device of claim 1, additionally comprising a multiwell plate having a plurality of wells formed therein;

said wells having openings dimensioned to produce an air tight seal around said fibre upon placement of said non-porous fibre in a well.

3. The device of claim 1 wherein the said guide or said positioning device is a component of a robotic system.

4. The device of claim 1, additionally comprising an agitator for:

agitating during contact of said non-porous fibres with said sample;

or

agitating when said non-porous fibres are submerged in the wells.

5. The device of claim 4 wherein said agitator is an orbital shaker, a sonicator, a vibrator or a stirrer.

6. The device of claim 1 wherein said positioning device is a component of an autosampler or a robotic system.

7. The device of claim 1 comprising more than one non-porous fibre per well having the same or different extraction phase.

8. The device of claim 1 wherein said polymeric extraction phase comprises substituted or unsubstituted poly (dimethylsiloxane), polyacrylate, poly (ethylene glycol), poly(divinyl benzene), ionic liquid or polypyrrole.

9. The device of claim 1 wherein the said extraction phase comprises a bioaffinity agent.

10. The device of claim 9 wherein said bioaffinity agent is selected from the group consisting of a selective cavity, a molecular recognition moiety, a molecularly imprinted polymer, and an immobilized antibody.

11. The device of claim 1, wherein the said non-porous fibres are made of metal, metal alloy or fused silica.

12. The device of claim 1, wherein said extraction phase is at least partially coated with a biocompatible protection layer.

13. The device of claim 12, wherein said protection layer is selected from poly (ethylene glycol), poly(hydroxyethyl methacrylate), poly(acrylamide), poly(N, N-di methyl acrylamide), dextran, poly(acrylonitrile), poly(urethane) and derivatized cellulose.

14. The device of claim 13 wherein the said extraction phase additionally comprises derivatized silica.

15. The device of claim 1 wherein the said extraction phase comprises a bioaffinity agent selected from the group consisting of a selective cavity, a molecular recognition moiety, a molecularly imprinted polymer, an immobilized antibody, and aptamers.

16. The device of claim 15 wherein said immobilized antibody is attached to metal or metal alloy wire coated with polyimide prepared by uniform coating of said polymer.

17. The device of claim 1, wherein said extraction phase comprises a mixture of derivatized silica and poly (ethylene glycol).

18. The device of claim 1, wherein said extraction phase contains a calibrant.

19. The device of claim 2 wherein said plurality of wells have two positions:

a closed position where the non-porous fibre is sealed in the well; and

an opened position where the fibre can be exposed to sample, desorption solvent or an analytical instrument.

20. The device of claim 1 wherein said non-porous fibre comprises a hollow distal end, and wherein an interior surface of the hollow distal end is coated with the extraction phase.

21. The device of claim 20 wherein said non-porous fibre comprises a needle having the extraction phase coated on the interior surface of the hollow distal end.

22. The device of claim 1 wherein said positioning device positions said non-porous fibres into an analytical instrument.

23. A solid-phase microextraction device for measuring or identifying one or more components of interest from liquid samples arranged in a plurality of wells in a multiwell plate, said device comprising:

a plurality of non-porous fibres, each having a distal end at least partially coated with a polymeric extraction phase for absorbing or adsorbing the component of interest from the liquid samples, said extraction phase being formed as a thin flat membrane in the shape of a fin on the distal end of the non-porous fibres; and

a positioning device for guiding the coated distal end of said non-porous fibres into a submerged position within the plurality of wells of a multiwell plate, and for removing said non-porous fibres from said wells;

said positioning device comprising;

(i) a mounting plate for mounting each of a plurality of non-porous fibres in a position corresponding to a counterpart well on said multiwell plate, the non-porous fibres being retractably mounted within the mounting plate; and

(ii) a guide for moving said mounting plate so the coated distal end of each of said non-porous fibres is submerged in a well of the multiwell plate, for retracting said non-porous fibres from said wells, and for positioning said non-porous fibres into a multiwell plate containing desorption solvent or into an analytical instrument.

24. The device of claim 23, additionally comprising a multiwell plate having a plurality of wells formed therein;

said wells having openings dimensioned to produce an air tight seal around said fibre upon placement of said non-porous fibre in a well.

25. The device of claim 23, wherein said guide or positioning device is a component of a robotic system.

26. The device of claim 23, additionally comprising an agitator for:

agitating during contact of said non-porous fibres with said sample;

or

agitating when said non-porous fibres are submerged in the wells.

27. The device of claim 26 wherein said agitator is an orbital shaker, a sonicator, a vibrator or a stirrer.

28. The device of claim 23 wherein said positioning device is a component of an autosampler or a robotic system.

29. The device of claim 23 comprising more than one non-porous fibre per well having the same or different extraction phase.

30. The device of claim 29 wherein said polymeric extraction phase comprises substituted or unsubstituted poly (dimethylsiloxane), polyacrylate, poly (ethylene glycol), poly(divinyl benzene), ionic liquid or polypyrrole.

31. The device of claim 23 wherein said extraction phase comprises a bioaffinity agent.

32. The device of claim 31 wherein said bioaffinity agent is selected from the group consisting of a selective cavity, a molecular recognition moiety, a molecularly imprinted polymer, and an immobilized antibody.

33. The device of claim 23, wherein said non-porous fibres are made of metal, metal alloy or fused silica.

34. The device of claim 23, wherein said extraction phase is at least partially coated with a biocompatible protection layer.

35. The device of claim 34, wherein said protection layer is selected from poly (ethylene glycol), poly(hydroxyethyl methacrylate), poly(acrylamide), poly(N, N-di methyl acrylamide), dextran, poly(acrylonitrile), poly(urethane) and derivatized cellulose.

36. The device of claim 35 wherein said extraction phase additionally comprises derivatized silica.

37. The device of claim 23 wherein said extraction phase comprises a bioaffinity agent selected from the group consisting of a selective cavity, a molecular recognition moiety, a molecularly imprinted polymer, an immobilized antibody, and aptamers.

38. The device of claim 37 wherein said immobilized antibody is attached to metal or metal alloy wire coated with polyimide prepared by uniform coating of said polymer.

39. The device of claim 23, wherein said extraction phase comprises a mixture of derivatized silica and poly (ethylene glycol).

40. The device of claim 23, wherein said extraction phase contains a calibrant.

41. The device of claim 24 wherein said plurality of wells have two positions:

a closed position where the non-porous fibre is sealed in the well; and

an opened position where the fibre can be exposed to sample, desorption solvent or an analytical instrument.

42. The device of claim 23 wherein said positioning device positions said non porous fibres into an analytical instrument.