Method for using a test card

• US 7,259,021 B2
• Filed: 03/06/2001
• Issued: 08/21/2007
• Est. Priority Date: 03/07/2000
• Status: Expired due to Fees

• Current Assignee: Bio Merieux
• Original Assignee: Bio Merieux
• Inventors: Colin, Bruno
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): HYUN, PAUL SANG HWA
• Application Number:
  US10/220,845
• Publication Number:
  US 20030148535A1
• Time in Patent Office: 2,359 Days
• Field of Search: 422/100, 436/180
• US Class Current: 436/180
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The present invention relates to a method for using a test card (1) comprising at least one fluid circuit, constituting a network of channels (3) and/or of compartments (17), for transferring at least one biological liquid from at least one inlet (2) to at least one outlet (6, 17), and a hydrophobic fluid is injected into the test card, into all or part of the network of said card, prior to the transfer of the biological liquid(s), said hydrophobic fluid filling the capillary spaces formed by the angles of the network of channels and/or of compartments of the card and avoiding the formation of bubbles and/or of dead volumes during the transfer of the biological liquid.

The invention is of preferential use in the field of microfluidics applied to biology.

Claims

1. A method of using a test card comprising at least one fluid circuit having capillary spaces, the method comprising the steps of:

injecting a hydrophobic fluid into the at least one fluid circuit constituting a network of at least one of channels or compartments, such that the entire network of channels is filled with the hydrophobic fluid;

removing any excess hydrophobic fluid with a second fluid such that only capillary spaces formed by angles of the network are filled with hydrophobic fluid, the hydrophobic fluid forming rounded edges, thereby avoiding formation of bubbles in the capillary spaces and/or of dead volumes during transfer of a biological liquid; and

transferring at least one biological fluid from at least one inlet to at least one outlet of the fluid circuit.

2. The method as claimed in claim 1, characterized in that the second fluid is a gas.

3. The method as claimed in claim 1, characterized in that a predetermined amount of hydrophobic fluid is injected into the card, this amount being sufficient to coat all or part of the fluid circuit in order to avoid the formation of bubbles and/or dead volumes.

4. The method as claimed in claim 1, characterized in that the hydrophobic fluid has a kinematic viscosity of between 1 and 100 centistokes at 40°

C.

5. The method as claimed in claim 1, characterized in that the hydrophobic fluid is a silylated oil.

6. The method as claimed in claim 2, wherein said gas is air.

7. The method as claimed in claim 4, wherein the hydrophobic fluid has a kinematic viscosity between 4 and 50 centistokes at 40°

C.

8. The method as claimed in claim 1, wherein the excess fluid is a volume of the hydrophobic fluid injected into the test card minus the volume of the hydrophobic fluid filling the capillary spaces.