Methods for treating neuropathic pain by administering IL-10 polypeptides

• US 7,261,882 B2
• Filed: 12/18/2003
• Issued: 08/28/2007
• Est. Priority Date: 06/23/2003
• Status: Active Grant

• Current Assignee: The Board of Regents of the University of Colorado (University of Colorado System)
• Original Assignee: University of Colorado System
• Inventors: Watkins, Linda May Rothblum
• Primary Examiner(s): Saoud; Christine J.
• Assistant Examiner(s): Lockard; Jon M
• Application Number:
  US10/742,641
• Publication Number:
  US 20040258671A1
• Time in Patent Office: 1,349 Days
• Field of Search: None
• US Class Current: 424/85.2
• CPC Class Codes: A61K 38/1793 for cytokines; for lymphokines; for interferons
• CPC Class Codes: A61K 38/1841 Transforming growth factor [TGF]
• CPC Class Codes: A61K 38/20 Interleukins [IL]
• CPC Class Codes: A61K 38/2026 IL-4
• CPC Class Codes: A61K 38/2066 IL-10
• CPC Class Codes: A61K 38/2086 IL-13 to IL-16
• CPC Class Codes: A61K 38/34 Melanocyte stimulating hormone [MSH], e.g. alpha- or beta-melanotropin
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61K 48/005 characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
• CPC Class Codes: A61K 48/0075 characterised by an aspect of the delivery route, e.g. oral, subcutaneous
• CPC Class Codes: A61K 48/0083 characterised by an aspect of the administration regime
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/04 Centrally acting analgesics, e.g. opioids
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
• CPC Class Codes: C07K 2319/32 fusions with soluble part of a cell surface receptor, "decoy receptors"
• CPC Class Codes: C07K 2319/75 containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2710/10343 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2750/14143 viral genome or elements thereof as genetic vector

Abstract

Methods of treating pain by delivery of anti-inflammatory cytokines, proinflammatory cytokine antagonists, and agents that act to reduce or prevent proinflammatory cytokine actions, to the nervous system are described. These agents can be delivered using gene therapy techniques. Alternatively, the agents can be delivered in protein compositions.

Claims

1. A method of treating existing neuropathic pain in a vertebrate subject that has been experiencing pain for at least 30 hours, comprising intrathecally administering to said subject a therapeutically effective amount of a composition comprising an interleukin-10 (IL-10) protein.

2. The method of claim 1, wherein the IL-10 is fused to the Fc portion of an IgG.

3. The method of claim 1, wherein said vertebrate subject is a human and said IL-10 is human IL-10.

4. The method of claim 1, wherein said vertebrate subject is a human and said IL-10 comprises the sequence of SEQ ID NO:

1.

5. A method of treating existing neuropathic pain in a vertebrate subject that has been experiencing pain for at least 30 hours, comprising administering to said subject a therapeutically effective amount of a composition comprising an interleukin-10 (IL-10) protein, or a bioloaically active fragment or active variant thereof, wherein said subject is administered said composition by intrathecal or epidural delivery.

6. The method of claim 5, wherein the IL-10 protein or fragment or variant is fused to the Fc portion of an IgG.

7. The method of claim 5, wherein said vertebrate subject is a human and said IL-10 is human IL-10.

8. The method of claim 5, wherein said IL-10 protein is a fragment of IL-10 comprising the sequence of SEQ ID NO:

4.

9. The method of claim 8, wherein said IL-10 protein consists of the sequence of SEQ ID NO:

4.