Enzymatic cascade bioreactor
First Claim
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1. A system for converting sucrose to, β
- -D-glucose, comprising;
a reservoir comprising a solution of sucrose;
pumping means in fluid communication with said reservoir;
a first porous packed column comprising invertase, said first porous packed column-in fluid communication with said pumping means;
a second porous packed column comprising glucose isomerase, said second porous packed column in fluid communication with said first porous packed column;
a third porous packed column comprising mutarotase, said third porous packed column in fluid communication with said second porous packed column; and
means for heating said first, second and third porous packed columns.
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Abstract
Disclosed is an apparatus and method for continuously converting sucrose to β-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and α-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.
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22 Claims
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1. A system for converting sucrose to, β
- -D-glucose, comprising;
a reservoir comprising a solution of sucrose; pumping means in fluid communication with said reservoir; a first porous packed column comprising invertase, said first porous packed column-in fluid communication with said pumping means; a second porous packed column comprising glucose isomerase, said second porous packed column in fluid communication with said first porous packed column; a third porous packed column comprising mutarotase, said third porous packed column in fluid communication with said second porous packed column; and means for heating said first, second and third porous packed columns. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
- -D-glucose, comprising;
Specification