Chimeric histone acetyltransferase polypeptides

• US 7,264,964 B2
• Filed: 06/21/2002
• Issued: 09/04/2007
• Est. Priority Date: 06/22/2001
• Status: Expired due to Fees

• Current Assignee: Ceres (Land O'Lakes Incorporated)
• Original Assignee: Ceres (Land O'Lakes Incorporated)
• Inventors: Dang, Van-Dinh, Okamuro, Jack
• Primary Examiner(s): Qian; Celine
• Assistant Examiner(s): DUNSTON, JENNIFER ANN
• Application Number:
  US10/177,478
• Publication Number:
  US 20030165903A1
• Time in Patent Office: 1,901 Days
• Field of Search: 536/23.4, 536/23.1, 536/23.5, 435/320.1
• US Class Current: 435/320.1
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: C12N 15/8267 Seed dormancy, germination or sprouting
• CPC Class Codes: C12N 15/829 Female sterility
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: C12N 9/14 Hydrolases (3)
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: G01N 33/6875 Nucleoproteins
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Chimeric polypeptides are disclosed that comprise a first polypeptide segment having histone acetyltransferase enzymatic activity and a second polypeptide segment that is similar to a subunit of a chromatin-associated histone deacetyltransferase protein complex. Also disclosed are nucleic acids encoding such chimeric polypeptides and eukaryotic organisms expressing such chimeric polypeptides.

Claims

1. An isolated nucleic acid construct encoding a chimeric polypeptide comprising

the amino acid sequence set forth in SEQ ID NO:

19.

3. The isolated nucleic acid of claim 1 or claim 2, wherein said isolated nucleic acid construct further comprises a regulatory element to facilitate expression of said encoded chimeric polypeptide.

4. The isolated nucleic acid of claim 3, wherein said regulatory element is a cell type specific or tissue-specific promoter.

2. An isolated nucleic acid construct encoding a chimeric polypeptide comprising

the amino acid sequence set forth in SEQ ID NO:

21.