Label-free methods for performing assays using a colorimetric resonant optical biosensor

• US 7,264,973 B2
• Filed: 09/22/2003
• Issued: 09/04/2007
• Est. Priority Date: 10/30/2000
• Status: Expired due to Term

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Lin, Bo, Li, Peter, Cunningham, Brian
• Primary Examiner(s): Chin; Christopher L.
• Application Number:
  US10/667,696
• Publication Number:
  US 20040132214A1
• Time in Patent Office: 1,443 Days
• Field of Search: None
• US Class Current: 436/518
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C40B 30/04 by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
• CPC Class Codes: C40B 30/10 by measuring physical properties, e.g. mass
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

The instant invention provides compositions and methods for determining cell interactions that are faster than conventional methods and that require the use of fewer reagents than conventional methods.

Claims

1. A method of detecting binding of one or more cells to one or more specific binding substances comprising:

(a) immobilizing one or more specific binding substances to two or more distinct locations on an internal surface of a vessel, wherein the internal surface of the vessel comprises a colorimetric resonant reflectance optical biosensor;

(b) illuminating the vessel with light;

(c) detecting one or more peak wavelength values (PWVs) for each distinct location;

(d) applying one or more cells to the internal surface of the vessel;

(e) illuminating the vessel with light;

(f) detecting one or more peak wavelength values (PWVs) for each distinct location;

(g) comparing the PWV'"'"'s of step (c) to the PWV'"'"'s of step (f);

wherein, if the one or more cells have bound to one or more specific binding substances, then the PWV is shifted at the distinct location to which the cells are bound.

2. The method of claim 1, wherein the vessel is a microtiter well, microtiter plate, test tube, petri dish or microfluidic channel.

3. The method of claim 1, wherein one or more specific binding substances are arranged in an array of distinct locations on the internal surface of the vessel that comprises a colorimetric resonant reflectance optical biosensor.

4. The method of claim 3, wherein the distinct locations define an array spot of about 50-500 microns in diameter.

5. The method of claim 1, wherein the one or more specific binding substances are immobilized on the internal surface of the vessel that comprises a colorimetric resonant reflectance optical biosensor by a method selected from the group consisting of physical adsorption, chemical binding, electrochemical binding, electrostatic binding, hydrophobic binding and hydrophilic binding.

6. The method of claim 1, wherein the specific binding substance is selected from the group consisting of nucleic acids, peptides, protein solutions, peptide solutions, single or double stranded DNA solutions, RNA solutions, RNA-DNA hybrid solutions, solutions containing compounds from a combinatorial chemical library, antigen, polyclonal antibody, monoclonal antibody, single chain antibody (scFv), F(ab) fragment, F(ab′

)₂ fragment, Fv fragment, small organic molecule, cell, virus, bacteria, polymer and biological sample.

7. The method of claim 1, wherein the one or more cells comprise more than one cell type.

8. The method of claim 1, wherein the one or more cells and the one or more specific binding substances do not comprise a detection label.