CE7-specific chimeric T cell receptor

• US 7,265,209 B2
• Filed: 06/30/2006
• Issued: 09/04/2007
• Est. Priority Date: 04/11/2001
• Status: Expired due to Term

• Current Assignee: City of Hope
• Original Assignee: City of Hope
• Inventors: Jensen, Michael
• Primary Examiner(s): Blanchard; David J.
• Application Number:
  US11/477,572
• Publication Number:
  US 20060246548A1
• Time in Patent Office: 431 Days
• Field of Search: 530/387.3, 530/388.3, 530/388.85, 435/372.3, 435/325, 435/328, 435/344, 435/344.1
• US Class Current: 530/387.3
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C07K 14/7051 T-cell receptor (TcR)-CD3 complex
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/03 containing a transmembrane segment
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

Genetically engineered, CE7-specific redirected immune cells expressing a cell surface protein having an extracellular domain comprising a receptor which is specific for CE7, an intracellular signaling domain, and a transmembrane domain, and methods of use for such cells for cellular immunotherapy of CE7+ neuroblastoma are disclosed. In one embodiment, the immune cell is a T cell and the cell surface protein is a single chain FvFc: ζ receptor where Fv designates the V_H and V_L chains of a single chain monoclonal antibody to CE7 linked by peptide, F_c represents a hinge-C_H2-C_H3 region of a human IgG₁, and ζ represents the intracellular signaling domain of the zeta chain of human CD3. DNA constructs encoding a chimeric T-cell receptor and a method of making a redirected T cell expressing a chimeric T cell receptor by electroporation using naked DNA encoding the receptor are also disclosed.

Claims

1. A CE7-specific chimeric T cell receptor comprising an intracellular signaling domain, a transmembrane domain and an extracellular domain, the extracellular domain comprising a CE7-specific receptor and wherein the CE7-specific chimeric T cell receptor comprises amino acids 21-631 of SEQ ID NO:

2.