Rapid flow fractionation of particles combining liquid and particulate dielectrophoresis

• US 7,267,752 B2
• Filed: 07/26/2005
• Issued: 09/11/2007
• Est. Priority Date: 07/28/2004
• Status: Expired due to Fees

• Current Assignee: University of Rochester
• Original Assignee: University of Rochester
• Inventors: King, Michael R., Jones, Thomas B., Ahmed, Rajib, Lomakin, Oleg
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US11/189,123
• Publication Number:
  US 20060108224A1
• Time in Patent Office: 777 Days
• Field of Search: 204/547, 204/643
• US Class Current: 204/547
• CPC Class Codes: B03C 5/005 Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength B03C5/02 takes precedence; electrophoresis B01D57/02
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]

Abstract

Rapid, size-based, deposition of particles from liquid suspension is accomplished using a nonuniform electric field created by coplanar microelectrode strips patterned on an insulating substrate. The scheme uses the dielectrophoretic force both to distribute aqueous liquid containing particles and, simultaneously, to separate the particles. Size-based separation is found within nanoliter droplets formed along the structure after voltage removal. Bioparticles or macromolecules of similar size can also be separated based on subtle differences in dielectric property, by controlling the frequency of the AC current supplied to the electrodes.

Claims

1. A method for selective separation of particles which are suspended in a liquid medium and which differ in regard to a characteristic, the method composing:

(a) applying the liquid medium to a pair of electrodes;

(b) applying a voltage to the pair of electrodes to generate a non-uniform electric field in the liquid medium, the voltage comprising an alternating-current voltage with a frequency sufficiently high for the electric field to penetrate through the liquid medium; and

(c) performing step (b) for a sufficient time that the liquid sample travels along the electrodes as a result of the voltage applied in step (b), with the particles which differ in regard to the characteristic having different spatial distributions along the electrodes.

2. The method of claim 1, further comprising (d) removing the voltage to cause the liquid sample to divide into droplets which are spaced along the electrodes.

3. The method of claim 1, wherein the electrodes are parallel electrodes.

4. The method of claim 3, wherein a dielectric material is disposed on the electrodes such that the dielectric material is between the electrodes and the liquid medium.

5. The method of claim 4, wherein a wetting control agent is disposed on the dielectric material such that the wetting control agent is disposed between the dielectric material and the liquid medium.

6. The method of claim 1, wherein the particles are biological particles.

7. The method of claim 6, wherein the biological particles are selected from the group consisting of cells, organelles, proteins, DNA, RNA, and combinations thereof.

8. The method of claim 7, wherein the biological particles are separated based on differences in dielectric properties.

9. The method of claim 1, wherein the characteristic comprises a size of the particles.

10. The method of claim 1, wherein the characteristic comprises a dielectric property of the particles.

11. A method for selective separation of particles which are suspended in a liquid medium and which differ in regard to a characteristic, the method comprising:

(a) applying the liquid medium to a pair of electrodes;

(b) applying a voltage to the pair of electrodes to generate a non-uniform electric field in the liquid medium, the voltage comprising an alternating-current voltage with a frequency sufficiently high for the electric field to penetrate through the liquid medium;

(c) performing step (b) for a sufficient time that the liquid sample travels along the electrodes, with the particles which differ in regard to the characteristic having different spatial distributions along the electrode; and

(d) removing the voltage to cause the liquid sample to divide into droplets which are spaced along the electrodes.

12. The method of claim 11, wherein the electrodes are parallel electrodes.

13. The method of claim 12, wherein a dielectric material is disposed on the electrodes such that the dielectric material is between the electrodes and the liquid medium.

14. The method of claim 13, wherein a wetting control agent is disposed on the dielectric material such that the wetting control agent is disposed between the dielectric material and the liquid medium.

15. The method of claim 11, wherein the particles are biological particles.

16. The method of claim 15, wherein the biological particles are selected from the group consisting of cells, organelles, proteins, DNA, RNA, and combinations thereof.

17. The method of claim 16, wherein the biological particles are separated based on differences in dielectric properties.

18. The method of claim 11, wherein the characteristic comprises a size of the particles.

19. The method of claim 11, wherein the characteristic comprises a dielectric property of the particles.